中国免疫学杂志2024，Vol.40Issue(10)：2158-2162,5.DOI:10.3969/j.issn.1000-484X.2024.10.023
CaMK4对狼疮性肾炎患者外周血中γδT细胞功能的影响
Effect of CaMK4 on γδT cells from peripheral blood in patients with lupus nephritis
王钟毓 1王洋彬 1雷飞飞 2李刚 2郗雪艳 3李方舟4
作者信息
- 1. 湖北医药学院第一临床医学院,十堰 442000
- 2. 湖北医药学院附属人民医院,十堰 442000
- 3. 湖北医药学院基础医学院,十堰 442000
- 4. 湖北医药学院生物医药研究院,十堰 442000
- 折叠
摘要
Abstract
Objective:To discussed the regulation role of calcium/calmodulin dependent protein kinase(CaMK4)on γδT cells in the pathogenesis of lupus nephritis.Methods:The proportion of γδT cells in peripheral blood of LN patients and healthy con-trols were compared by flow cytometry.Magnetic bead was used to separation LN patients and healthy controls of gamma delta T cells in peripheral blood.RNA-SEQ analysis were performed in γδT cells of peripheral blood from LN patients and healthy controls.Real-time PCR and western blot were used to verify the expression level of differential gene CaMK4.The expression levels of CD80,CD86 and CD40L on γδT cells treated with CaMK4 inhibitor were detected by flow cytometry.ELISA was used to measure the level of IL-17 secreted by γδT cells after CaMK4 inhibitor treatment.Results:The proportion of γδT cells in the peripheral blood of LN patients was lower than that of healthy control group.A total of 28 differential genes related to LN were screened by RNA-SEQ sequencing,among which CaMK4 had significant by differences at molecular level and protein level.After γδT cells from LN patients were treated with CaMK4 inhibitor,KN-93,the expressions of surface costimulatory molecules CD80,CD86 and CD40L was decreased,and the level of IL-17 also was decreased.Conclusion:CaMK4 regulates γδT cells to participate in the pathogenesis of LN by IL-17 secretion and the expression of costimulatory molecules.Inhibition of CaMK4 may become a new target for the treatment of LN.关键词
γδT细胞/狼疮性肾炎/CaMK4/IL-17Key words
γδT cells/Lupus nephritis/CaMK4/IL-17分类
医药卫生引用本文复制引用
王钟毓,王洋彬,雷飞飞,李刚,郗雪艳,李方舟..CaMK4对狼疮性肾炎患者外周血中γδT细胞功能的影响[J].中国免疫学杂志,2024,40(10):2158-2162,5.
