中国中医药信息杂志2024，Vol.31Issue(10)：121-128,8.DOI:10.19879/j.cnki.1005-5304.202311645
复方地黄颗粒对帕金森病阴虚动风证大鼠肠-肝-脑轴DJ-1、IP3R、GRP75、VDAC1表达的影响
Effects of Compound Dihuang Granules on the Expressions of DJ-1,IP3R,GRP75 and VDAC1 in Gut-Liver-Brain Axis in Rats with Parkinson Disease with Yin Deficiency and Wind Movement Syndrome
摘要
Abstract
Objective To observe the effect of compound Dihuang Granules on the expressions of DJ-1,IP3R,GRP75 and VDAC1 in liver and colonic tissue of Parkinson disease(PD)rats with yin deficiency and wind movement syndrome.Methods 6-Hydroxydopamine was used to inject into the right substantia nigra to prepare PD with yin deficiency and wind movement syndrome rats model.65 rats that successfully modeled were randomly divided into model group,madopar group(madopar 150 mg/kg)and TCM low-,medium-and high-dosage groups(compound Dihuang Granules 1.75,3.5,7 g/kg),with 13 rats in each group;another 13 rats without intervention were selected as the normal control group,and 13 rats were only injected with ascorbic acid solution as sham-operation group.The administration groups were given corresponding drugs by gavage,and the normal control group,sham-operation group and model group were given normal saline by gavage,once a day for 28 consecutive days.The neurobehavioral changes of rats were observed,and the morphology of liver tissue and colonic tissue were observed by HE staining,the protein and mRNA expressions of DJ-1,IP3R,GRP75 and VDAC1 in liver and colonic tissue were detected by immunohistochemical staining,Western blot and RT-PCR,respectively.Results Compared with the normal control group and the sham-operation group,the number of rotation circles of rats in the model group increased,with granular degeneration of liver tissue,loose cytoplasm of cells,slight hepatic steatosis,edema of colonic mucosal and submucosal layers,loose connective tissue,infiltration of a small number of lymphocytes,and significant vascular dilation,the expressions of DJ-1,IP3R,GRP75 and VDAC1 protein and mRNA in liver and colonic tissue were significantly decreased(P<0.01).Compared with the model group,the number of rotation circles of rats in madopar group and TCM groups were significantly decreased,with mild granular degeneration of liver cells,loose cytoplasm,orderly arrangement of hepatic cords,mild edema of the colon,a small amount of lymphocyte and granulocyte infiltration,and a small amount of vascular dilation,the protein and mRNA expressions of DJ-1,IP3R,GRP75 and VDAC1 in liver and colonic tissue significantly increased(P<0.05).The TCM high-dosage group showing the most obvious changes,there was no statistical significance with the madopar group(P>0.05).Conclusion Compound Dihuang Granules may restore endoplasmic reticulum-mitochondria homeostasis,improve gut-liver-brain axis mitochondrial function,protect dopamine neurons,and delay PD by regulating the expressions of DJ-1,IP3R,GRP75 and VDAC1 in liver tissue and colonic tissue.关键词
帕金森病/阴虚动风证/复方地黄颗粒/肠-肝-脑轴/大鼠Key words
Parkinson disease/yin deficiency and wind movement syndrome/compound Dihuang Granules/gut-liver-brain axis/rats分类
医药卫生引用本文复制引用
王行玲,孙光洁,吕子微,何建成,梁建庆..复方地黄颗粒对帕金森病阴虚动风证大鼠肠-肝-脑轴DJ-1、IP3R、GRP75、VDAC1表达的影响[J].中国中医药信息杂志,2024,31(10):121-128,8.基金项目
国家自然科学基金(82160929) （82160929）
甘肃省科技厅第十一批省级科技计划自然科学基金(自筹经费)项目(21JR11RA143) （自筹经费）
敦煌医学与转化教育部重点实验室开放课题(DHYX18-08) （DHYX18-08）
甘肃中医药大学中医学一级学科"岐黄英才"导师专项基金硕导项目(ZYXKSD-202211) （ZYXKSD-202211）
