中国中西医结合外科杂志2024，Vol.30Issue(5)：628-632,5.DOI:10.3969/j.issn.1007-6948.2024.05.004
缺氧诱导SD大鼠阴茎海绵体平滑肌细胞内炎症反应及番茄红素的干预作用
Hypoxia-induced inflammatory response in penile corpora cavernosal smooth muscle cells of SD rats and the interventional role of lycopen
摘要
Abstract
Objective To explore the hypoxia-induced inflammatory response in corpus cavernosum smooth muscle cells(CCSMCs)of SD rats and the regulatory effect of lycopene.Methods Primary CCSMCs were isolated from SD rats and observed for morphological stability up to the fourth passage.Immunofluorescence staining for α-smooth muscle actin(α-SMA)and desmin was performed to verify the purity of cultured CCSMCs.After 24 hours of exposure to different concentrations of CoCl2 and lycopene,either alone or in combination,cell viability was assessed using the Cell Counting Kit-8(CCK8).The experiment included three treatment groups:control,hypoxia(200 μmol/LCoCl2),and lycopene treatment(40 μmol/L).Western blotting and reverse transcription quantitative polymerase chain reaction(RT-qPCR)were used to detect the levels of hypoxia-inducible factor 1-alpha(HIF-1α),tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),interleukin-18(IL-18),and interleukin-1 beta(IL-1β)after 24 hours of culture.Results The CCSMCs maintained morphological uniformity up to the fourth passage.High purity was confirmed through positive staining for α-SMA and desmin.Following 200 pmol/L CoCl2 treatment,CCSMCs viability decreased to 50％,with increases in intracellular levels of HIF-1α,TNF-α,IL-6,IL-18(P＜0.05),and a significant rise in IL-1 β mRNA expression(P＜0.05),though protein levels showed only an increasing trend(P＞0.05).Lycopene at 40μmol/L elevated CCSMC viability to 80％of normal levels and significantly reduced HIF-1α,TNF-α,IL-18 protein expression,and TNF-α,IL-18,IL-1β mRNA levels(P＜0.05).IL-6 protein,IL-6 mRNA,and IL-1βprotein levels showed a decreasing trend(P＞0.05).Conclusion Under hypoxic conditions,the expression of inflammatory factors in CCSMCs is upregulated,and lycopene can effectively inhibit the expression of these inflammatory factors.关键词
缺氧/海绵体平滑肌细胞/炎症因子/番茄红素Key words
Hypoxia/corpus cavernosum smooth muscle cells/inflammatory factors/lycopene分类
医药卫生引用本文复制引用
张鑫,富一家,黄文杰,吕伯东..缺氧诱导SD大鼠阴茎海绵体平滑肌细胞内炎症反应及番茄红素的干预作用[J].中国中西医结合外科杂志,2024,30(5):628-632,5.基金项目
浙江省医学会临床医学科研专项资金(2023ZYC-A06) （2023ZYC-A06）
