中国中西医结合外科杂志2024，Vol.30Issue(5)：633-636,4.DOI:10.3969/j.issn.1007-6948.2024.05.005
前列腺癌相关成纤维细胞通过分泌趋化因子C-C基元配体15促进单核细胞迁移
Fibroblasts associated with prostate cancer promote monocyte migration by secreting C-C motif chemokine ligand 15
摘要
Abstract
Objective To investigate the expression and origin of C-C motif chemokine ligand 15(CCL15)in the microenvironment of prostate cancer,as well as its impact on monocyte migration ability.Methods CCL15 immunofluorescence staining was performed on normal prostate tissue and prostate cancer tissue,and immunohistochemical and immunofluorescence double staining experiments were used to explore the main source cells of CCL15.Isolate fibroblasts from fresh normal prostate tissue and prostate cancer tissue and perform primary culture.Detect fibroblasts through qPCR,Western βlotting and ELISA experiments expression of α-SMA,FAP,Vimentin and CCL15.Using lentivirus transfection to knock down CCL15,investigate the effect of knocking down CCL15 on the migration ability of monocytes in fibroblast conditioned medium.Perform multi-color immunofluorescence staining to detect the positional relationship between CCL15 and M2 macrophages in prostate cancer tissue.Results The expression of CCL15 in prostate cancer tissue is significantly higher than that in normal tissue,and it mainly comes from its expression of α-SMA.Compared with normal fibroblasts,prostate cancer associated fibroblasts significantly enhance their ability to secrete CCL15.After knocking down CCL15,the secretion of CCL15 by cancer associated fibroblast was significantly reduced,weakening the migration ability of monocytes.In prostate cancer tissue,there are more M2 macrophages in the high expression region of CCL15.Conclusion CAF promotes monocyte migration and increases M2 macrophage infiltration in the prostate cancer microenvironment by secreting CCL15.关键词
前列腺癌/成纤维细胞/单核细胞/免疫微环境/趋化因子C-C基元配体15Key words
Prostate cancer/fibroblasts/monocytes/immune microenvironment/C-C motif chemokine ligand 15分类
医药卫生引用本文复制引用
邵毅,宋圣举,韩瑞发,尚芝群..前列腺癌相关成纤维细胞通过分泌趋化因子C-C基元配体15促进单核细胞迁移[J].中国中西医结合外科杂志,2024,30(5):633-636,4.基金项目
天津市泌尿外科研究所人才资助计划(MYSRC202307) （MYSRC202307）
天津市教委科研计划项目(自然科学)(2023ZD004) （自然科学）
天津市科技特派员项目(23YDTPJC00180) （23YDTPJC00180）
天津市医院协会项目(2022ZC13) （2022ZC13）
