中国中西医结合外科杂志2024，Vol.30Issue(5)：637-642,6.DOI:10.3969/j.issn.1007-6948.2024.05.006
微小染色体维持蛋白复合物6、细胞分裂周期相关蛋白6在肾透明细胞癌组织中的表达及其与恶性生物学行为的相关性研究
MCM6/CDC6 is a potential biomarker for renal clear cell carcinoma
杨拓 1蔡科科 1赵朋 1王金铸 1刘立朋 1马际春1
作者信息
- 1. 天津市中西医结合医院泌尿外科||天津市急腹症器官损伤与中西医修复重点实验室(天津 300100)
- 折叠
摘要
Abstract
Objective To explore the correlation between the expression of MCM6,CDC6 and malignant biological behavior in renal clear cell carcinoma tissues.Methods 77 cases of renal clear cell carcinoma tissue confirmed by pathology were used as the study group,and homologous distal normal renal tissue was used as the control group for the MCM6 and CDC6 study;Based on the GEPIA database,the expression of MCM6 and CDC6 in renal clear cell carcinoma tissue and normal tissue was compared using monoclonal antibody immunohistochemistry technology;qRT-PCR and Western Blotting were used to detect the effect of shRNA on MCM6 expression:CCK-8 and colony formation assay were used to detect the in vitro proliferation results of HTB-47 and CRL-1932 cell lines.Results Compared with normal renal tissue,MCM6,CDC6 was significantly upregulated in renal clear cell carcinoma tissue,and upregulation of MCM6 was associated with reduced disease-free survival rate(P＜0.05);Knocking down the MCM6 gene of HTB-47 and CRL-1932 cell lines can significantly inhibit the proliferation of tumor cells and significantly block the tumor cell division cycle staying in the G2/M phase;The expression of MCM6 was significantly correlated with the volume size of renal cell carcinoma(P=0.010),and according to the GEPIA database,there was a significant correlation between CDC6 and MCM6(P＜0.01).All expression results were further validated by immunohistochemical staining.Conclusion The upregulation of MCM6 expression is related to the malignant behavior and clinical progression of renal clear cell carcinoma,which can regulate cell proliferation by affecting the cell cycle.In renal clear cell carcinoma tissues,the expression levels of MCM6 and CDC6 are significantly upregulated and closely related,indicating that MCM6/CDC6 may become a potential biomarker for predicting renal clear cell carcinoma and a new therapeutic target for targeted therapy of this disease.关键词
微小染色体维持蛋白复合物6/细胞分裂周期相关蛋白6/肾透明细胞癌/生物标记物Key words
Minichromosome maintenance complex component 6/cell division cycle 6/renal clear cell carcinoma/biomarker分类
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杨拓,蔡科科,赵朋,王金铸,刘立朋,马际春..微小染色体维持蛋白复合物6、细胞分裂周期相关蛋白6在肾透明细胞癌组织中的表达及其与恶性生物学行为的相关性研究[J].中国中西医结合外科杂志,2024,30(5):637-642,6.
