肿瘤药学2024，Vol.14Issue(4)：442-447,6.DOI:10.3969/j.issn.2095-1264.2024.04.09
卷柏总双黄酮有效部位通过抑制PI3K/AKT通路抗弥漫大B细胞淋巴瘤
The effective parts of total biflavones from selaginella inhibit diffuse large B-cell lymphoma by down-regulating PI3K/AKT pathway
摘要
Abstract
Objective To explore the effects and mechanism of total biflavonoids(TBF)effective parts extracted from Se-laginella tamariscina on diffuse large B-cell lymphoma(DLBCL).Methods CCK-8 method was used to detect the effects of TBF effective parts on the proliferation of DLBCL cells.Flow cytometry was applied to detect the apoptosis and cell cycle of cells after treated by TBF effective parts.Transcriptome sequencing and enrichment of differential genes were conducted to preliminarily explore the mechanism of TBF effective parts in anti-DLBCL.Western blotting was used to verify the signaling pathways affected by TBF effective parts.Results The TBF effective parts significantly inhibited the proliferation of different types of DLBCL cell lines,induced an increase in the proportion of apoptosis of DLBCL cells,and blocked the cell cycle in G2 phase.The TBF effective parts mainly affected the intracellular PI3K-AKT signaling pathway.Compared with the control group,the expression of PI3K and AKT,as well as their phosphorylated forms(p-PI3K and p-AKT),were significantly decreased in a TBF effective parts concentration-dependent manner.Conclusion The TBF effective parts played an anti-DLBCL role by inhib-iting the activation of the PI3K/AKT signaling pathway.TBF effective parts may serve as a new option for the treatment of DLBCL.关键词
卷柏/总双黄酮/弥漫大B细胞淋巴瘤/凋亡/PI3K/AKT信号通路Key words
Selaginella/Total biflavonoids/DLBCL/Apoptosis/PI3K/AKT signaling pathway分类
医药卫生引用本文复制引用
苏畅,肖玲,周辉,徐康平,卢桂阁,胡倩宇,朱雪婷,王彩琴,贺怡子,曾若兰,李亚军..卷柏总双黄酮有效部位通过抑制PI3K/AKT通路抗弥漫大B细胞淋巴瘤[J].肿瘤药学,2024,14(4):442-447,6.基金项目
湖南省中医药管理局一般项目(D2022074) （D2022074）
湖南省卫生健康委一般项目(202203045455) （202203045455）
湖南省自然科学基金(2022JJ30026) （2022JJ30026）
湖南省自然科学基金(2022JJ30789) （2022JJ30789）
中南大学中央高校基础研究基金(2023ZZTS0867) （2023ZZTS0867）
中南大学中央高校基础研究基金(2024ZZTS0918) （2024ZZTS0918）
湖南省卫健委技术厅资助项目(WZ2020-13) （WZ2020-13）
长沙血液病精准诊疗技术创新中心([2024]NO.196). （[2024]NO.196）
