肿瘤药学2024，Vol.14Issue(4)：448-456,9.DOI:10.3969/j.issn.2095-1264.2024.04.10
KIF2C在乳腺癌中的表达及其对肿瘤生长的影响
Expression of KIF2C in breast cancer and its effect on tumor growth
摘要
Abstract
Objective To investigate the expression of kinesin family member 2C(KIF2C)in breast cancer and its ef-fects on the proliferation,migration and invasion of breast cancer cells.Methods The data of breast cancer patients in GSE1456,GSE9195,GSE4922 and GSE96058 datasets of the Cancer Genome Atlas(TCGA)and Gene Expression Omni-bus(GEO)database were collected and analyzed by bioinformatics methods to identify the key gene KIF2C and to predict the expression and prognostic value of KIF2C in breast cancer.The expression of KIF2C protein in 39 cases of breast can-cer tissues and adjacent tissues was detected by immunohistochemistry.qPCR and JESS automatic protein analyzer were used to detect the mRNA and protein expression of KIF2C in normal breast cells MCF10A and breast cancer cell lines MCF7,T47D,SK-BR-3,MDA-MB-231 and BT474.The cell lines with high mRNA and protein expression were selected for lentivirus infection to silence KIF2C.Immunofluorescence was used to detect the expression of Ki-67.Transwell meth-od was used to detect the migration and invasion ability of cells,and flow cytometry was used to detect the cell cycle.Re-sults A total of 661 differentially-expressed genes(DEGs)in breast cancer were collected from the TCGA-BRCA database.After intersected with GSE1456,GSE9195,GSE4922 and GSE96058 datasets,37 breast cancer recurrence-related prog-nostic DEGs were obtained.Five key genes were identified by RAA algorithm,and KIF2C was preliminarily analyzed by bioinformatics.Immunohistochemistry results showed that KIF2C was highly expressed in breast cancer tissues.The results of qRT-PCR and JESS automatic protein analyzer showed that the expression level of KIF2C in breast cancer cells MDA-MB-231 was significantly higher than that in normal breast cells MCF10A.Silencing KIF2C could inhibit the proliferation,migration and invasion of MDA-MB-231 cells,and block the S phase of cell cycle.Conclusion KIF2C is highly expressed in breast cancer tissues and cells,and silencing KIF2C can inhibit the proliferation,migration,and invasion of breast can-cer cells.关键词
乳腺癌/KIF2C/增殖/侵袭/迁移Key words
Breast cancer/KIF2C/Proliferation/Invasion/Migration分类
医药卫生引用本文复制引用
麦孜悦,曾健..KIF2C在乳腺癌中的表达及其对肿瘤生长的影响[J].肿瘤药学,2024,14(4):448-456,9.基金项目
广西科技计划项目(桂科AB18126054). （桂科AB18126054）
