浙江医学2024，Vol.46Issue(18)：1918-1924,7.DOI:10.12056/j.issn.1006-2785.2024.46.18.2023-2398
川芎嗪对脂多糖处理大鼠肾小管上皮细胞S1PR3的抑制作用
Inhibitory effect of ligustrazine on S1PR3 of rat renal tubular epithelial cells treated with lipopolysaccharide
摘要
Abstract
Objective To investigate the effects of ligustrazine on sphingosine-1-phosphate receptor(S1PR)3 in rat renal tubule epithelial cells treated by lipopolysaccharide(LPS),and the role and mechanism of S1PR3 in sepsis-associated acute kidney injury(S-AKI).Methods The cultured NRK-52E cells were divided into control group,LPS group,and ligustrazine+LPS group.The control group received no treatment;LPS group was pretreated with 0.9％sodium chloride solution equivalent to ligustrazine for 30 min,and then cells were collected by centrifugation for examination after being stimulated with LPS(20 μg/mL)for 12 h.Ligustrazine+LPS group was pretreated with ligustrazine 200 ng/mL for 30 min,and then cells were collected by centrifugation for examination after being stimulated with LPS 20 μg/mL for 12 h.Flow cytometry was used to detect the differences of apoptosis rate,calcium ion expression and Caspase-3 expression in all groups,and ELISA was used to detect the differences of Calpain 1 and Calpain 2 expressions.The expression of S1PR3 in cells of the three groups was detected by Western blot.Results The NRK-52E cell model of sepsis was successfully established after LPS treatment.Compared with the control group,the apoptosis rate,calcium ion expression,Caspase-3 expression,S1PR3 expression,and Calpain 1 and Calpain 2 expressions in LPS group were significantly increased(all P＜0.01).Compared with LPS group,apoptosis rate,calcium ion concentration,Caspase-3 expression,S1PR3 expression,Calpain 1 and Calpain 2 expressions in ligustrazine+LPS group were significantly decreased(all P＜0.05).Conclusion Ligustrazine can inhibit the expression of S1PR3 and decrease the concentration of calcium ions in renal tubular epithelial cells,thereby inhibiting the apoptosis signaling pathway of Caspase-3 cells and reducing the apoptosis of renal tubular cells.S1PR3 may play an important role in the occurrence and development of S-AKI.关键词
脓毒症/急性肾损伤/川芎嗪/1-磷酸鞘氨醇受体3/肾小管上皮细胞Key words
Sepsis/Acute kidney injury/Ligustrazine/Sphingosine-1-phosphate receptor 3/Renal tubular epithelial cells引用本文复制引用
吴晋,应静..川芎嗪对脂多糖处理大鼠肾小管上皮细胞S1PR3的抑制作用[J].浙江医学,2024,46(18):1918-1924,7.基金项目
浙江省医药卫生科技计划项目(2020KY250) （2020KY250）
宁波市自然科学基金项目(2017A610192) （2017A610192）
