川芎嗪对脂多糖处理大鼠肾小管上皮细胞S1PR3的抑制作用OACSTPCD
Inhibitory effect of ligustrazine on S1PR3 of rat renal tubular epithelial cells treated with lipopolysaccharide
目的 探讨川芎嗪对脂多糖(LPS)处理大鼠肾小管上皮细胞(NRK-52E)中1-磷酸鞘氨醇受体(S1PR)3的影响以及S1PR3在脓毒症相关急性肾损伤(S-AKI)中的作用及机制.方法 将培养好的大鼠NRK-52E细胞分为对照组、LPS组、川芎嗪+LPS组.对照组不予处理;LPS组予川芎嗪等量0.9％氯化钠溶液预处理30 min后,予LPS 20 μg/mL刺激12 h后离心收集细胞待检;川芎嗪+LPS组先用川芎嗪200 ng/mL预处理30 m…查看全部>>
Objective To investigate the effects of ligustrazine on sphingosine-1-phosphate receptor(S1PR)3 in rat renal tubule epithelial cells treated by lipopolysaccharide(LPS),and the role and mechanism of S1PR3 in sepsis-associated acute kidney injury(S-AKI).Methods The cultured NRK-52E cells were divided into control group,LPS group,and ligustrazine+LPS group.The control group received no treatment;LPS group was pretreated with 0.9％sodium chloride solution equival…查看全部>>
吴晋;应静
315010 宁波大学附属第一医院麻醉科315010 宁波大学附属第一医院麻醉科
脓毒症急性肾损伤川芎嗪1-磷酸鞘氨醇受体3肾小管上皮细胞
SepsisAcute kidney injuryLigustrazineSphingosine-1-phosphate receptor 3Renal tubular epithelial cells
《浙江医学》 2024 (18)
1918-1924,7
浙江省医药卫生科技计划项目(2020KY250)宁波市自然科学基金项目(2017A610192)
评论