植物学报2024，Vol.59Issue(5)：738-751,14.DOI:10.11983/CBB24065
水稻抗氧化性状遗传位点挖掘及候选基因分析
Genetic Locus Mining and Candidate Gene Analysis of Antioxidant Traits in Rice
摘要
Abstract
Rice(Oryza sativa)is one of the most important food crops in the world.Improving its antioxidant ability and stress resistance is an important way to ensure high and stable yields.In this study,we used the indica rice HZ and the japonica rice Nekken2 as parents and the 120 recombinant inbred line population constructed from them as experimental materials to determine the hydroxyl radical scavenging rate,total phenol content,flavonoid content,and anthocyanin content in sword leaves,glume shells and grains of parents and their progeny at three stages:the tillering stage,the grain filling stage and the maturity stage.Additionally,a total of 62 QTLs related to rice antioxidant damage were identified on the basis of the constructed high-density genetic map for QTL mapping,with an LOD value of up to 4.36.A quantitative analysis of candidate genes related to antioxidant damage ability in these regions revealed that thirteen candidate genes,including LOC_Os06g01850,LOC_Os12g07820,LOC_Os12g07830,and LOC_Os03g60509 were significantly differen-tially expressed between the two parents at different growth stages.A multitude of QTLs associated with antioxidant damage resistance in rice were identified,providing a foundation for further mapping and cloning of related genes and the development of new rice varieties with increased resistance and nutritional value.关键词
水稻/抗氧化性/遗传图谱/QTL定位Key words
rice/antioxidant ability/genetic map/QTL mapping引用本文复制引用
连锦瑾,徐昕璇,吴日成,路梅,王长春,饶玉春,唐璐瑶,张伊诺,郑佳兴,朱超宇,叶语涵,王跃星,商文楠,傅正浩..水稻抗氧化性状遗传位点挖掘及候选基因分析[J].植物学报,2024,59(5):738-751,14.基金项目
浙江省自然科学基金重点项目(No.LZ23C130003)、2024年国家级大学生创新创业训练计划项目和2024年浙江省大学生科技创新活动计划暨新苗人才计划 （No.LZ23C130003）
