植物学报2024，Vol.59Issue(5)：774-782,9.DOI:10.11983/CBB24107
植物基因组学中的流式细胞分析及分选技术
Flow Cytometric Analysis and Sorting in Plant Genomics
摘要
Abstract
Flow cytometry is a high-throughput technology that allows for the simultaneous and rapid detection of multiple physical and biological characteristics of individual particles.With the significant reduction in sequencing costs,flow cy-tometry is playing an increasingly prominent role in high-throughput sample acquisition for plant genomics.Taking rice and soybean as examples,this paper describes in detail the application of flow cytometry for fine sorting of plant cell nuclei and the subsequent ATAC-seq and RNA-seq experiments and analysis process,which provides a preferred tool for efficient mining of genes for agrobiological breeding.The key techniques and common problems in the experimental ope-ration,such as the precautions for cell nuclei preparation,the balance between sorting purity and efficiency,and the de-bugging method for single-cell sorting,were also analyzed and suggested to provide references for the plant scientists in applying flow cytometry to carry out genomics research.关键词
植物/流式细胞术/细胞核/分选/ATAC-seq/RNA-seqKey words
plants/flow cytometry/nuclei/sorting/ATAC-seq/RNA-seq引用本文复制引用
夏春皎,李运广,夏姝,庞伟,陈春丽..植物基因组学中的流式细胞分析及分选技术[J].植物学报,2024,59(5):774-782,9.基金项目
国家重点研发计划(No.2023ZD04073)和华中农业大学研究生培养条件建设(No.2024SC03) （No.2023ZD04073）
