植物学报2024，Vol.59Issue(5)：783-791,9.DOI:10.11983/CBB24063
花粉管细胞壁原子力显微镜观测制样方法优化
Optimization of Preparation and Detection Methods for Pollen Tube Cell Wall by Atomic Force Microscopy
摘要
Abstract
Atomic force microscopy(AFM)is a powerful tool for studying the ultrastructure and mechanical properties of plant cell walls,while good sample preparation is essential for AFM data acquisition.As micron-scale plant samples,pollen tubes are typical experimental materials for studying the structure and function of cell walls.Due to the difficulty in sample preparation,it is hard to obtain in situ AFM data in physiological state of pollen tubes as pollen tubes are hard to attach to the substrate firmly in fluid.In this study,the AFM preparation and detection methods are optimized using Nico-tiana tabacum pollen tubes as the experimental materials.Thin solid medium is used as the adhesive,thereby pollen tubes are attached to the glass slide during germination and elongation in fluidic environment.Compared with the con-ventional drying-rehydration method,the optimized liquid-attaching method allows observation directly in fluid without drying treatment,which avoids the shrinkage and denaturation of pollen tubes caused by drying-rehydration process.Accordingly,liquid-attaching method can be applied for pollen tubes of different species and sizes,providing support to obtain high-resolution in situ AFM data of pollen tube cell walls in native physiological state under aqueous conditions.关键词
原子力显微镜/花粉管/细胞壁/原位/力学性质Key words
atomic force microscope/pollen tube/cell wall/in situ/mechanical properties引用本文复制引用
覃思颖,罗燕,张禾,胡君,廖菊够..花粉管细胞壁原子力显微镜观测制样方法优化[J].植物学报,2024,59(5):783-791,9.基金项目
国家自然科学基金(No.32060175,No.32060043)和云南省基础研究计划重点项目(No.202201AS070014) （No.32060175,No.32060043）
