植物学报2024，Vol.59Issue(5)：792-799,8.DOI:10.11983/CBB24051
栽培薯蓣茎段离体再生体系的建立
Establishment of an In Vitro Regeneration System for Stem Segments of Cultivated Dioscorea polystachya
摘要
Abstract
To establish a tissue culture regeneration system for cultivated Chinese yam(Dioscorea polystachya),the effects of different concentrations of plant growth regulators,and stem segments on axillary bud induction and plant re-generation were investigated with different concentrations of plant growth regulators,media types,and shoot tips and stem segments at four different positions under the shoot tip as explants.The results revealed that MS+1.0 mg·L-16-BA+0.5 mg·L-1 KT hormone ratio and 12-20 cm stem segment under the stem tip were the best formulas for the induc-tion of adventitious buds in D.polystachya,and the induction rate reached 90.0％.In the subculture,the optimal concen-tration was MS+0.5 mg·L-1 6-BA+0.05 mg·L-1 NAA+0.1 mg·L-1 KT,and the proliferation coefficient increased with in-creasing of 6-BA within a certain range when the low concentration of NAA was constant.With the same concentration of exogenous hormones,DKW medium was significantly better than the conventional 1/2MS medium,and the rooting rate is significantly improved,reaching 92.86％.Adding 1 mg PVP(polyvinylpyrrolidone)and increasing the number of transfers can significantly reduce browning.This study effectively solved the problem of optimizing the explant position for the sta-ble of in vitro propagation of cultivated yam germplasm resources,laying a good foundation for the large-scale production of their high-quality virus-free seedlings.关键词
栽培薯蓣/茎段/组织培养/离体再生Key words
Dioscorea polystachya/stem segments/tissue culture/in vivo regeneration引用本文复制引用
冯雯,王玉国..栽培薯蓣茎段离体再生体系的建立[J].植物学报,2024,59(5):792-799,8.基金项目
上海市崇明区农业科创项目(No.2021CNKC-01-02) （No.2021CNKC-01-02）
