栽培薯蓣茎段离体再生体系的建立OA北大核心CSTPCD
Establishment of an In Vitro Regeneration System for Stem Segments of Cultivated Dioscorea polystachya
为建立栽培薯蓣(Dioscorea polystachya)组培再生体系,以茎尖及茎尖下4个不同长度的茎段为外植体,探讨了不同浓度植物生长调节剂、培养基类型及茎段对腋芽诱导和植株再生的影响.结果表明,培养基配比为MS+1.0 mg·L-16-BA+0.5 mg·L-1 KT和茎尖下12-20 cm的茎段为栽培薯蓣不定芽诱导的最佳组合,诱导率达90.0％;继代培养最适培养基配方为MS+0.5 mg·L-1 6-BA+0.05 mg·L-1 NAA+…查看全部>>
To establish a tissue culture regeneration system for cultivated Chinese yam(Dioscorea polystachya),the effects of different concentrations of plant growth regulators,and stem segments on axillary bud induction and plant re-generation were investigated with different concentrations of plant growth regulators,media types,and shoot tips and stem segments at four different positions under the shoot tip as explants.The results revealed that MS+1.0 mg·L-16-BA+0.5…查看全部>>
冯雯;王玉国
复旦大学生命科学学院,上海 200438复旦大学生命科学学院,上海 200438
栽培薯蓣茎段组织培养离体再生
Dioscorea polystachyastem segmentstissue culturein vivo regeneration
《植物学报》 2024 (5)
792-799,8
上海市崇明区农业科创项目(No.2021CNKC-01-02)
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