植物学报2024，Vol.59Issue(5)：800-809,10.DOI:10.11983/CBB23152
北美豆梨杂种幼胚挽救及再生体系的建立
Establishment of Immature Embryo Rescue and Regeneration System for Pyrus calleryana cv.'Cleveland'
摘要
Abstract
In this study,the seed embryos of Pyrus calleryana cv.'Cleveland'were used as materials to systematically study the sterilization of seed embryo explants,callus induction,adventitious bud proliferation and rooting medium.The results showed that the germination rate of young embryos stored at 4℃ for 21 d was the highest(67.23％)The suitable sterilization treatment of seed embryo explants was 75％alcohol(30 s)+10％H2O2(10 min)+0.1％HgCl2(14 min).The most suitable medium for seed embryo germination was 1/2MS+4.0 mg·L-1 6-BA+0.5 mg·L-1 NAA,and the germination rate was 89.67％.The optimum culture medium for callus induction was 1/2MS+1.0 mg·L-1 IBA+1.0 mg·L-1 6-BA,and the callus induction rate was 93.33％.The best differentiation medium was 1/2MS+0.2 mg·L-1 IBA+2.0 mg·L-1 6-BA,and the regeneration frequency was 87.44％.Best transgenerational proliferation medium was 1/2MS+1.5 mg·L-1 6-BA+0.1 mg·L-1 IBA,and the proliferation rate was 100％.The most suitable medium for root formation was 1/2MS+20 g·L-1 su-crose+1.0 g·L-1 active carbon+1.5 mg·L-1 IBA+0.05 mg·L-1 NAA,and the rooting rate was 82.63％.In summary,the seed embryo regeneration system of P.calleryang cv.'Cleveland'was established,which provides scientific basis and guidan-ce for the efficient reproduction of high-quality pear germplasm resources.关键词
豆梨/胚挽救/组织培养/再生体系Key words
Pyrus calleryana cv.'Cleveland'/embryo rescue/tissue culture/regeneration system引用本文复制引用
刘玉泽,王一菲,任威蓁,栗浩,路斌,路丙社,于晓跃..北美豆梨杂种幼胚挽救及再生体系的建立[J].植物学报,2024,59(5):800-809,10.基金项目
河北农业大学引进人才科研专项(No.702/3118141)、河北省自然科学基金(No.C2021204126)和河北省自然科学青年基金(No.C2024204207) （No.702/3118141）
