中国中医药信息杂志2024，Vol.31Issue(10)：39-45,7.DOI:10.19879/j.cnki.1005-5304.202309540
基于网络药理学及实验验证探讨达原饮治疗幽门螺杆菌阳性口疮作用机制
Exploration on the Mechanism of Dayuan Decoction in Treating Hp Positive Oral Ulcer Based on Network Pharmacology and Experimental Validation
摘要
Abstract
Objective To explore the mechanism of Dayuan Decoction in treating Helicobacter pylori(Hp)positive oral ulcer based on network pharmacology and experimental validation.Methods Active components of Dayuan Decoction and its targets were collected using the TCMSP database;relevant targets for Hp positive oral ulcer were screened through the GeneCards database;the Venny 2.1.0 online platform was used to screen the intersection targets of drug active components and diseases;a protein-protein interaction network was built using STRING 11.0 database and core key targets were screened using Cytoscape 3.7.1 software;GO and KEGG enrichment analysis was conducted on intersection targets using the DAVID database.Oral administration of standard Hp bacterial solution and burning of oral mucosa with glacial acetic acid were used to construct an animal model of Hp positive oral ulcer,and the model rats were intervented with Dayuan Decoction by gavage.The morphology of oral and gastric mucosal tissues was observed,and the expression of core targets in oral and gastric mucosal tissues were detected.Results A total of 165 targets were obtained for the treatment of Hp positive oral ulcer with Dayuan Decoction.The main active components were quercetin,kaempferol,5,8,2'-trihydroxy-7-methoxyflavone,etc.,the core targets were AKT1,IL6,TP53,TNF,etc.GO and KEGG enrichment analysis suggested that Dayuan Decoction in the treatment of Hp positive oral ulcer was mainly related to the inflammatory pathway.Animal experiments showed that compared with the blank group,the structure of the oral and gastric tissues in the model group rats were significantly disrupted,with significant infiltration of inflammatory cells;the contents of IL-6 and TNF-α in the serum significantly increased(P<0.05),the contents of TLR4 and NLRP3 in oral and gastric mucosal tissues significantly increased(P<0.05),and the protein expression of TLR4 and NF-κB in oral mucosal tissues significantly increased(P<0.05).Compared with the model group,the histopathological manifestations of rats in all doseage of Dayuan Decoction groups significantly improved,the contents of IL-6 and TNF-α in the serum,and the contents of TLR4 and NLRP3 in the oral and gastric mucosal tissues significantly decreased,the protein expression of TLR4 and NF-κB in the oral mucosal tissue significantly decreased(P<0.05).Conclusion Dayuan Decoction is possible to regulate the inflammatory development of Hp positive oral ulcer and improve their symptoms by downregulating TLR4 and NF-κB levels.关键词
网络药理学/达原饮/幽门螺杆菌阳性口疮/实验验证/作用机制/大鼠Key words
network pharmacology/Dayuan Decoction/Hp positive oral ulcer/experimental verification/mechanism/rats分类
医药卫生引用本文复制引用
易园园,张平,夏习林,刘雪峰..基于网络药理学及实验验证探讨达原饮治疗幽门螺杆菌阳性口疮作用机制[J].中国中医药信息杂志,2024,31(10):39-45,7.基金项目
湖北省自然科学基金创新发展联合基金项目(2024AFD293) （2024AFD293）
