浙江农林大学学报2024，Vol.41Issue(5)：1037-1046,10.DOI:10.11833/j.issn.2095-0756.20240110
黄槽毛竹叶绿体基因组及毛竹种下分类群的叶绿体基因组序列比较
Chloroplast genome of Phyllostachys edulis f.luteosulcata and comparison of chloroplast genome sequence of subspecies of Ph.edulis
摘要
Abstract
[Objective]This study aims to sequence,assemble,annotate,and analyze the chloroplast genome of Phyllostachys edulis f.luteosulcata.The research also involves comparing its chloroplast genetic information and phylogenetic relationships with those of other subspecies of Ph.edulis(moso bamboo).[Method]High-throughput sequencing data were used to assemble and annotate the complete chloroplast genome of Ph.edulis f.luteosulcata.Subsequently,we analyze the composition,codon preference,and repetitive sequences of the genome.Furthermore,sequence comparison and phylogenetic analysis were conducted to compare the phylogenetic relationships and genome sequence differences among different subspecies of moso bamboo.[Result]The chloroplast genome of Ph.edulis f.luteosulcata is a double-loop DNA of 139 678 bp in length containing 132 genes,including 85 protein-coding genes,eight ribosomal RNAs(rRNAs),and 39 transfer RNAs(tRNAs).The codon preference for this genome has an A/U base at the end.There are 49 repetitive sequences with the most common type being A/T and 55 Simple Sequence Repeat(SSR)sites.Phylogenetic analyses constructed using the chloroplast genome sequences showed that Ph.edulis f.luteulosulcata is in a monophyletic branch together with other subspecies of Ph.edulis and is closely related to the original variety of Ph.edulis var.pubescens.The analysis of chloroplast genome sequence and coding gene characteristics showed that there were differences in the number and structure of coding genes and low degree of sequence variation among the subspecies of Ph.edulis.[Conclusion]This study is the first to comparatively analyze the chloroplast genomes of subspecies of Ph.edulis and reveals a degree of sequence variation in these subspecies of Ph.edulis.This variation information would be available for the identification and comparison of subspecies of Ph.edulis.[Ch,5 fig.2 tab.27 ref.]关键词
黄槽毛竹/叶绿体基因组/系统进化/序列变异Key words
Phyllostachys edulis f.luteosulcata/chloroplast genome/phylogenetics/sequence variation分类
生物科学引用本文复制引用
刘萱,邹龙海,周明兵..黄槽毛竹叶绿体基因组及毛竹种下分类群的叶绿体基因组序列比较[J].浙江农林大学学报,2024,41(5):1037-1046,10.基金项目
国家自然科学基金资助项目(31870656) （31870656）
浙江省自然科学基金重点项目(LZ19C160002) （LZ19C160002）
