中国中西医结合杂志2024，Vol.44Issue(9)：1079-1085,7.DOI:10.7661/j.cjim.20240729.051
解毒祛瘀滋肾方对强的松所致MRL/lpr小鼠脂质代谢紊乱的调控作用
Regulatory Effects of Jiedu Quyu Zishen Recipe on Prednisone-Induced Lipid Metabolism Disorders in MRL/lpr Mice
摘要
Abstract
Objective investigate the impact of glucocorticoids on lipid metabolism in a systemic lupus erythematosus(SLE)mouse model,and the regulatory effect of Jiedu Quyu Zishen Recipe(JQZR)on lipids.Methods Totally 12 MRL/lpr mice were randomly divided into the model group,Western medicine group(10 mg/kg prednisone),and combined therapy group(10 mg/kg prednisone+20 g/kg JQZR),with 4 mice in each group.Blood samples were collected at 10 and 30 days post-treatment.Serum lipid profiles were analyzed using Shotgun technique,followed by principal component analysis after unit variance scaling.Subsequently,non-parametric tests were applied to identify lipids with significant differences among groups.Results Compared with the model group,treatment with 10 mg/kg prednisone for 10 and 30 days led to significant changes in the lipid profile.After 10 days of prednisone treatment,24 kinds of lipids in the serum of MRL/lpr mice showed significant differences(P＜0.05),and after 30 days of treatment,more lipids(51 kinds)were affected.After 10 days of combined treatment with JQZR and prednisone,16 out of 24 lipids returned to the level of the model group.Following 30 days of combined treatment,25 out of 51 lipids returned to the level of the model group.Conclusion Prednisone can interfere with lipid metabolism in MRL/lpr mice,and JQZR has a significant regulatory effect on lipid disorders,which may be one of the mechanisms of alleviating the side effects of glucocorticoids.关键词
系统性红斑狼疮/鸟枪法技术/脂质组学/脂质代谢/糖皮质激素/解毒祛瘀滋肾方/中药复方Key words
systemic lupus erythematosus/Shotgun technique/lipidomics/lipid metabolism/glucocorticoid/Jiedu Quyu Zishen Recipe/Chinese herbal compound引用本文复制引用
吴德鸿,张夏风,郭翊煊,尹梦迪,周佳..解毒祛瘀滋肾方对强的松所致MRL/lpr小鼠脂质代谢紊乱的调控作用[J].中国中西医结合杂志,2024,44(9):1079-1085,7.基金项目
浙江省自然科学基金联合基金项目(No.LBY22H270003) （No.LBY22H270003）
浙江省自然科学基金资助项目(No.LY22H290006) （No.LY22H290006）
国家自然科学基金资助项目(No.81703966) （No.81703966）
浙江省中医药科技计划项目(No.2022ZB171) （No.2022ZB171）
浙江中医药大学校级科研项目(No.2022JKZKTS43) （No.2022JKZKTS43）
