中国中西医结合杂志2024，Vol.44Issue(9)：1094-1099,6.DOI:10.7661/j.cjim.20240301.036
姜黄素抑制甲基硝基亚硝基胍诱导胃黏膜上皮细胞上皮间质转化
Curcumin Inhibits Epithelial-Mesenchymal Transition in Human Gastric Epithelial Cells Induced by N-methyl-N'-nitro-N-nitrosoguanidine
摘要
Abstract
Objective To explore the inhibitory effect and underlying mechanism of curcumin(Cur)on epithelial-mesenchymal transition(EMT)in human gastric epithelial cells(GES-1)induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG).Methods The GES-1 cells were divided into the control group,the MNNG treatment group(the concentration of MNNG action was 20 µmol/L,the stably passaged cells obtained in this group were used as MC1 cells),the curcumin treatment group(the concentration of Curcumin action was 20 µmol/L),and the MNNG+curcumin group(the stably passaged cells obtained in this group were used as MC2 cells).The impact of various concentrations of curcumin on GES-1 cell proliferation was assessed using the MTT assay.The influence of curcumin intervention on the morphology and growth of MNNG-induced gastric mucosal epithelial cells was observed under an optical microscope.Cell migration ability was evaluated using the scratch healing method.The expression levels of PTEN and Vimentin were detected by Western Blot and immunofluorescence assays.Results MTT assay results indicated that curcumin concentrations ranging from 5-20 µmol/L showed no significant impact on GES-1 cell growth and proliferation.However,higher concentrations of curcumin were found to inhibit the proliferation of GES-1 cells.MNNG was observed to induce EMT in GES-1 cells,and curcumin significantly affected the morphological changes associated with MNNG-induced EMT.The scratch repair assay revealed that compared with the MC1 cells,the scratch healing speed of MC2 cells exhibited a significant deceleration at 48 h and 72 h(P＜0.05).Subsequent Western Blot and immunofluorescence analyses unveiled that compared with the MNNG group,PTEN expression was up-regulated in the MNNG+curcumin group and the expression of the mesenchymal marker Vimentin was down-regulated in the MNNG+curcumin group and the curcumin treatment group(P＜0.05).PTEN expression in EMT-transformed MC1 cells was significantly lower than that in MC2 cells(P＜0.05).Conclusion Curcumin could inhibit the MNNG-induced EMT process in GES-1 cells,and the mechanism might be related to the relevant signaling pathway mediated by PTEN.关键词
姜黄素/上皮间质转化/胃癌/人第10号染色体缺失的磷酸酶及张力蛋白同源基因/中药提取物Key words
curcumin/epithelial-mesenchymal transition/gastric cancer/phosphatase and tensin homolog deleted on chromosome ten/Chinese herbal extracts引用本文复制引用
贾婷婷,万学婷,潘秋如,覃月凤,强占荣..姜黄素抑制甲基硝基亚硝基胍诱导胃黏膜上皮细胞上皮间质转化[J].中国中西医结合杂志,2024,44(9):1094-1099,6.基金项目
广西自然科学基金项目(No.2022GXNSFAA103007) （No.2022GXNSFAA103007）
桂林市科技开发项目(No.20210227-8-4) （No.20210227-8-4）
桂林医学院中青年教职工科研能力提升项目(No.2018glmcy091) （No.2018glmcy091）
