Transcriptomic and epigenomic landscapes of muscle growth during the postnatal period of broilersOACSTPCD
Background Broilers stand out as one of the fastest-growing livestock globally,making a substantial contribution to animal meat production.However,the molecular and epigenetic mechanisms underlying the rapid growth and development of broiler chickens are still unclear.This study aims to explore muscle development patterns and regulatory networks during the postnatal rapid growth phase of fast-growing broilers.We measured the growth performance of Cornish(CC)and White Plymouth Rock(RR)over a 42-d period.Pectoral muscle samples from both CC and RR were randomly collected at day 21 after hatching(D21)and D42 for RNA-seq and ATAC-seq library construction.Results The consistent increase in body weight and pectoral muscle weight across both breeds was observed as they matured,with CC outpacing RR in terms of weight at each stage of development.Differential expression analysis identified 398 and 1,129 genes in the two dimensions of breeds and ages,respectively.A total of 75,149 ATAC-seq peaks were annotated in promoter,exon,intron and intergenic regions,with a higher number of peaks in the promoter and intronic regions.The age-biased genes and breed-biased genes of RNA-seq were combined with the ATAC-seq data for subsequent analysis.The results spotlighted the upregulation of ACTC1 and FDPS at D21,which were primarily associated with muscle structure development by gene cluster enrichment.Additionally,a noteworthy upregulation of MUSTN1,FOS and TGFB3 was spotted in broiler chickens at D42,which were involved in cell differentiation and muscle regeneration after injury,suggesting a regulatory role of muscle growth and repair.Conclusions This work provided a regulatory network of postnatal broiler chickens and revealed ACTC1 and MUSTN1 as the key responsible for muscle development and regeneration.Our findings highlight that rapid growth in broiler chickens triggers ongoing muscle damage and subsequent regeneration.These findings provide a foundation for future research to investigate the functional aspects of muscle development.
Shuang Gu;Qiang Huang;Yuchen Jie;Congjiao Sun;Chaoliang Wen;Ning Yang;
State Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding,China Agricultural University,Beijing 100193,China National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics,Breeding and Reproduction,Ministry of Agriculture and Rural Affairs,Department of Animal Genetics and Breeding,College of Animal Science and Technology China Agricultural University,Beijing 100193,China.State Key Laboratory of Animal Biotech Breeding and Frontier Science Center for Molecular Design Breeding,China Agricultural University,Beijing 100193,China National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics,Breeding and Reproduction,Ministry of Agriculture and Rural Affairs,Department of Animal Genetics and Breeding,College of Animal Science and Technology China Agricultural University,Beijing 100193,China. Sanya Institute of China Agricultural University,Hainan 572025,China
畜牧业
ATAC-seqBroilerPectoral muscle developmentRapid growthRNA-seq
《Journal of Animal Science and Biotechnology》 2024 (005)
P.1851-1865 / 15
supported by the National Key Research and Development Program of China(2022YFF1000204);the National Natural Science Foundation of China(32102535);the Key Research and Development Program of Hainan province(ZDYF2023XDNY036)。
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