Cadherin-18 loss in prospermatogonia and spermatogonial stem cells enhances cell adhesion through a compensatory mechanismOACSTPCD
Extracellular membrane proteins are crucial for mediating cell attachment,recognition,and signal transduction in the testicular microenvironment,particularly germline stem cells.Cadherin 18(CDH18),a type Ⅱ classical cadherin,is primarily expressed in the nervous and reproductive systems.Here,we investigated the expression of CDH18in neonatal porcine prospermatogonia(ProSGs)and murine spermatogonial stem cells(SSCs).Disruption of CDH18 expression did not adversely affect cell morphology,proliferation,self-renewal,or differentiation in cultured porcine ProSGs,but enhanced cell adhesion and prolonged cell maintenance.Transcriptomic analysis indicated that the down-regulation of CDH18 in ProSGs significantly up-regulated genes and signaling pathways associated with cell adhesion.To further elucidate the function of CDH18 in germ cells,Cdh18 knockout mice were generated,which exhibited normal testicular morphology,histology,andspermatogenesis.Transcriptomic analysis showed increased expression of genes associated with adhesion,consistent with the observations in porcine ProSGs.The interaction of CDH18withβ-catenin and JAK2 in both porcine ProSGs and murine SSCs suggested an inhibitory effect on the canonical Wnt and JAK-STAT signaling pathways during CDH18 deficiency.Collectively,these findings highlight the crucial role of CDH18 in regulating cell adhesion in porcine ProSGs and mouse SSCs.Understanding this regulatory mechanism provides significant insights into the testicular niche.
Xiao-Xiao Li;Dan-Chen Zhang;Yan Wang;Jian Wen;Xing-Ju Wang;Yu-Lu Cao;Ru Jiang;Jia-Rui Li;Yi-Nuo Li;He-He Liu;Wen-Hai Xie;Zheng-Feng Xu;Ping Hu;Kang Zou
Germline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China Stem Cell Research and Translation Center,Nanjing Agricultural University,Nanjing,Jiangsu 210095,ChinaGermline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China Stem Cell Research and Translation Center,Nanjing Agricultural University,Nanjing,Jiangsu 210095,ChinaDepartment of Prenatal Diagnosis,Women’s Hospital of Nanjing Medical University,Nanjing Maternity and Child Health Care Hospital,Nanjing,Jiangsu 210004,ChinaGermline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China Stem Cell Research and Translation Center,Nanjing Agricultural University,Nanjing,Jiangsu 210095,ChinaGermline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China Stem Cell Research and Translation Center,Nanjing Agricultural University,Nanjing,Jiangsu 210095,ChinaGermline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China Stem Cell Research and Translation Center,Nanjing Agricultural University,Nanjing,Jiangsu 210095,ChinaGermline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,ChinaGermline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,ChinaGermline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,ChinaGermline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,ChinaSchool of Life Sciences and Medicine,Shandong University of Technology,Zibo,Shandong 255000,ChinaDepartment of Prenatal Diagnosis,Women’s Hospital of Nanjing Medical University,Nanjing Maternity and Child Health Care Hospital,Nanjing,Jiangsu 210004,ChinaDepartment of Prenatal Diagnosis,Women’s Hospital of Nanjing Medical University,Nanjing Maternity and Child Health Care Hospital,Nanjing,Jiangsu 210004,ChinaGermline Stem Cells and Microenvironment Lab,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China Stem Cell Research and Translation Center,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China
生物学
Extracellular signalFertilityQuiescencyWnt signaling pathway
《Zoological Research》 2024 (5)
P.1048-1060,13
supported by the Jiangsu Seed Industry Revitalization Project(JBGS[2021]024)Fundamental Research Funds for the Central Universities(KYT2023002)Jiangsu Province Capability Improvement Project through Science,Technology,and Education Jiangsu Provincial Medical Key Discipline(ZDXK202211)Shandong Province Natural Science Foundation(ZR2020MC074)。
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