Bacteria colonization and gene expression related to immune function in colon mucosa is associated with growth in neonatal calves regardless of live yeast supplementationOACSTPCD
Background As Holstein calves are susceptible to gastrointestinal disorders during the first week of life,understanding how intestinal immune function develops in neonatal calves is important to promote better intestinal health.Feeding probiotics in early life may contribute to host intestinal health by facilitating beneficial bacteria colonization and developing intestinal immune function.The objective of this study was to characterize the impact of early life yeast supplementation and growth on colon mucosa-attached bacteria and host immune function.Results Twenty Holstein bull calves received no supplementation(CON)or Saccharomyces cerevisiae boulardii(SCB)from birth to 5 d of life.Colon tissue biopsies were taken within 2 h of life(D0)before the first colostrum feeding and 3 h after the morning feeding at d 5 of age(D5)to analyze mucosa-attached bacteria and colon transcriptome.Metagenome sequencing showed that there was no difference inαandβdiversity of mucosa-attached bacteria between day and treatment,but bacteria related to diarrhea were more abundant in the colon mucosa on D0 compared to D5.In addition,q PCR indicated that the absolute abundance of Escherichia coli(E.coli)decreased in the colon mucosa on D5 compared to D0;however,that of Bifidobacterium,Lactobacillus,and Faecalibacterium prausnitzii,which could competitively exclude E.coli,increased in the colon mucosa on D5 compared to D0.RNA-sequencing showed that there were no differentially expressed genes between CON and SCB,but suggested that pathways related to viral infection such as“Interferon Signaling”were activated in the colon mucosa of D5 compared to D0.Conclusions Growth affected mucosa-attached bacteria and host immune function in the colon mucosa during the first 5 d of life in dairy calves independently of SCB supplementation.During early life,opportunistic pathogens may decrease due to intestinal environmental changes by beneficial bacteria and/or host immune function.Predicted activation of immune function-related pathways may be the result of host immune function development or suggest other antigens in the intestine during early life.Further studies focusing on the other antigens and host immune function in the colon mucosa are required to better understand intestinal immune function development.
Koki Nishihara;Clothilde Villot;Lautaro Cangiano;Le Luo Guan;Michael Steele;
Department of Animal Biosciences,Animal Science and Nutrition,University of Guelph,Guelph,ON N1G 1Y2,CanadaDepartment of Agricultural,Food and Nutritional Science,University of Alberta,Edmonton T6G 2P5,Canada Lallemand SAS,Blagnac F‑31702,FranceDepartment of Animal and Dairy Sciences,University of Wisconsin-Madison,Madison,WI,USAFaculty of Land and Food Systems,The University of British Columbia,Vancouver,BC V6T 1Z4,CanadaDepartment of Animal Biosciences,Animal Science and Nutrition,University of Guelph,Guelph,ON N1G 1Y2,Canada Present Address:Department of Animal Biosciences,University of Guelph,Guelph,ON N1G 2W1,Canada
畜牧业
Colon biopsyDairy calvesRNA-sequencingSaccharomyces cerevisiae boulardii16S rRNA amplicon-sequencing
《Journal of Animal Science and Biotechnology》 2024 (005)
P.1980-1995 / 16
supported by funding from Lallemand Health Solution(Mirabel,QC);Alberta Milk(Edmonton,AB);the Saskatoon Colostrum Co.Ltd.(Saskatoon,SK);the Natural Sciences and Engineering Research Council of Canada(Ottawa,ON);supported by a Mitacs Accelerate Program from Mitacs Canada(Toronto,ON);Lallemand SAS(Blagnac,France)。
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