不同剂型岩藻黄素产品在Caco-2细胞模型中的吸收转运研究OA

[Objective]This study aims to investigate the comparative bioavailability of various formulations of fucoxanthin,a unique algal-derived carotenoid known for its antioxidant,weight management,and lipid-regulat-ing properties.Given fucoxanthin's inherent instability,its current applications predominantly rely on two formats:fucoxanthin microcapsule powder and fucoxanthin oil suspension.[Methods]To assess the bioavail-ability differences,the study employed a Caco-2 cell model to examine the transport and absorption dynamics of both fucoxanthin formulations alongside their respective gastrointestinal simulated digesta.The Caco-2 cell model was validated through assessments of cellular morphology,transepithelial electrical resistance,alkaline phosphatase activity,and permeability characteristics.Key metrics evaluated included sample transport efficacy,absorption rates,apparent permeability coefficients,and intracellular antioxidant capacity.[Results]Following 21-day culture,Caco-2 cells displayed a tight junctional architecture indicative of differentiation,rendering them suitable for absorption and transport studies.The findings revealed that the fucoxanthin microcapsule powder exhibited superior absorption efficiency at 27.98%,with a transport efficiency of 24.45%post 12-hour incuba-tion and an optimal apparent permeability coefficient of 8.1×10-5 cm/s observed within three hours,highlight-ing robust bioavailability.In contrast,the fucoxanthin oil suspension digestion solution achieved an absorption rate of 22.29%,with peak transport efficiency and permeability coefficient values of 43.91%and 1.4×10-5 cm/s,respectively,occurring after six hours.Additionally,both fucoxanthin samples demonstrated potent antioxidant effects within Caco-2 cells,notably augmenting superoxide dismutase(SOD)and catalase(CAT)activities.[Conclusion]Gastrointestinal digestion processes facilitate the formation of fucoxanthin micelles,thereby en-hancing its bioavailability.The microcapsule formulation maintained high stability and exhibited commendable absorption and transport capabilities following digestion,whereas the oil suspension format showcased elevated transmural transport efficiency post-digestion but compromised absorption performance.In practical applica-tions,the appropriate dosage form of fucoxanthin should be selected based on the product type and its character-istics.[Significance]These insights offer a foundational platform for future fucoxanthin product development and formulation strategies,as well as guidance on incorporation into nutraceuticals.