微嗜酸寡养单胞菌A2用于饲料添加剂的潜力分析OA北大核心CSTPCD

[Objective]To study the toxic effects of Stenotrophomonas acidaminiphila A2 fermentation broth on Kumming mice,and evaluate its safety aim to provide a theoretical reference for the develop-ment of feed additives for removing aflatoxin B1(AFB1).[Method]Kumming mice were given 0.02 mL·g-1·d-1 body weight of Stenotrophomonas acidaminiphila A2 fermentation broth daily for 14 days,and normal saline was used as the control group.Many index were evaluated,such as physical indicators by measuring growth performance,organ weight index,blood routine and serum biochemical indicators,antioxidant indicators in liver and kidney tissues(total antioxidant activity,superoxide dis-mutase,glutathione peroxidase and malondialdehyde),immune indicators of liver(IL-β and TNF-α)and spleen(CD3,CD4,CD8 and CD4/CD8),and liver histopathological sections.On this basis,the comprehensive evaluation is carried out.[Results]The results showed that Stenotrophomonas acidami-niphila A2 fermentation broth had no significant difference from the control group in growth perform-ance,organ index,blood routine,serum biochemical indicators,antioxidant indices of liver and kid-ney tissues,immune indices of liver and spleen,and histopathological sections of liver.[Conclusion]The results indicated that Stenotrophomonas acidaminiphila strain A2 fermentation broth had no obvious toxic effect on mice,and also had great potential as a feed additive.