好氧甲烷氧化系统氧氟沙星共代谢效能与微生物特征OA北大核心CSTPCD

Ofloxacin(OFL)belongs to the quinolone antibiotics,which is widely used in the fields of disease control,livestock and poultry breeding,etc.Its large amount of use leads to the presence of residual OFL in the natural environment.Methane oxidising bacteria can achieve biodegradation of refractory organics through Co-metabolism while using methane as a growth substrate.In this study,an aerobic methane oxidation co-metabolism system was constructed for OFL-containing wastewater,and the OFL removal efficiency and the corresponding microbial characteristics were investigated.The results showed that the aerobic methane oxidation system was able to achieve the effective degradation of OFL,and the average removal rate of OFL could reach 80.15％.Fourier infrared spectroscopy analysis showed that the system could effectively remove nitrogen and phosphorus pollutants and aromatic compounds.Metagenomics analysis showed that there were 15 species of methane-oxidizing bacteria at genus level,the main bacteria are aerobic methane oxidizing bacteria,with a total abundance of 0.30％-0.56％;157 functional genes related to methane metabolism were detected,and the dominant functional genes were E2.2.1.1/tktA/tktB,glyA/SHMT and xoxF;6 metabolic pathways were annotated at KEGG pathway level 1,and 47 metabolic pathways were annotated at KEGG pathway level 2.Drug metabolism-other enzymes,drug metabolism-cytochrome P450,and metabolism of xenobiotics by cytochrome P450 could promote the degradation of OFL.The results can provide a theoretical basis and technical support for the pollution prevention of antibiotics and similar compounds.