山东科学2024，Vol.37Issue(5)：1-9,94,10.DOI:10.3976/j.issn.1002-4026.20230175
基于细胞膜色谱技术的鹿角胶肽段筛选及鉴定
To explore the pharmacodynamic substance basis of Cervi Cornus Colla based on cell membrane chromatography
摘要
Abstract
Cell membrane chromatography/ultrahigh-phase liquid chromatography-mass spectrometry(CMC/UPLC-MS)was used to screen the active ingredients,i.e.,peptides,in the traditional Chinese medicine tablet Cervi cornus Colla,and the obtained spectra were analyzed using Maxquant software,Perseus software,and Uniprot database.Structures of these peptides were identified using Protein Data Bank,and their molecular properties such as their biological activity,adverse reactions,relative molecular mass,isoelectric point,and stability index were predicted using a bioinformatics platform.With an activity probability of 0.09,the peptides were identified as nontoxic,nonhemolytic,sensitizing,and highly hydrophilic,with a relative molecular mass of 1 541.68,a peptide chain length of 14,an isoelectric point of 3.92,and an instability index of 34.39.This method provides a feasible research approach for rapidly screening and identifying active ingredients(e.g.,peptides and proteins)that exhibit pharmacological effects.关键词
鹿角胶/细胞膜色谱/骨髓间充质干细胞/白细胞介素-1βKey words
Cervi Cornus Colla/cell membrane chromatography/bone marrow mesenchymal stem cells/interleukin-1β分类
中医学引用本文复制引用
孙铁锋,赵玉,王平,丁相龙,丁立钧,王金国..基于细胞膜色谱技术的鹿角胶肽段筛选及鉴定[J].山东科学,2024,37(5):1-9,94,10.基金项目
济南市"新高校20条"(202333006) （202333006）
山东省中医药科技项目(2020Q023) （2020Q023）
山东省自然科学基金(ZR2020MH386) （ZR2020MH386）
国家重点研发计划政府间国际科技创新合作项目(2019YFE0117800) （2019YFE0117800）
中央引导地方科技发展专项基金(YDZX2021117) （YDZX2021117）
山东省重点研发计划(科技示范工程)2021SFGC1205 （科技示范工程）
山东省中央引导地方科技发展资金项目(YDZX20203700002055) （YDZX20203700002055）
