山东科学2024，Vol.37Issue(5)：10-16,7.DOI:10.3976/j.issn.1002-4026.20230173
基于UHPLC-Q-Exactive Orbitrap-MS技术的垂盆草化学成分表征与鉴定
Characterization and identification of chemical constituents in Sedum sarmentosum Bung based on UHPLC-Q-Exactive Orbitrap-MS technology
摘要
Abstract
To develop an ultra-performance liquid chromatograpy-quadrupole electrostatic field orbitrap high-resolution mass spectrometry(UHPLC-Q-Exactive Orbitrap-MS)method for characterization and identification of chemical constituents in Sedum sarmentosum Bung.The separation was performed using a Phenomenex Kinetex C18 column(2.1 mm×100 mm,2.6 μm)with a mobile phase comprising acetonitrile and water containing 0.1％formic acid via gradient elution at a flow rate of 0.3 mL/min;the injection volume was 3.0 μL.Mass spectrometry was performed in positive and negative ionization modes with electrospray ionization.The chemical constituents were characterized and identified based on retention time,precise molecular weight,fragment ions,and comparison with the reference substances.The results of this study showed that 53 compounds were characterized and identified in Sedum sarmentosum Bung,including 25 flavonoids,12 megastigmanes,11 organic acids,3 alkaloids and 2 other compounds.Five of these compounds were identified via comparison with reference compounds,and three compounds may have not been reported from Sedum sarmentosum Bung.The UHPLC-Q-Exactive Orbitrap-MS method proposed in this study can rapidly and comprehensively characterize the chemical constituents in Sedum sarmentosum Bung and provide a reference basis for the research on quality control and atherapeutic material basis of Sedum sarmentosum Bung.关键词
垂盆草/超高效液相-四级杆-静电场轨道阱高分辨质谱法/化学成分/黄酮Key words
Sedum sarmentosum Bung/UHPLC-Q-Exactive Orbitrap-MS/chemical constituents/flavonoids分类
中医学引用本文复制引用
朱心仪,杨纯国,田海涛,侯苗,韩利文,邓志鹏..基于UHPLC-Q-Exactive Orbitrap-MS技术的垂盆草化学成分表征与鉴定[J].山东科学,2024,37(5):10-16,7.基金项目
山东省中医药科技项目(M-2023191) （M-2023191）
山东省医药卫生科技发展计划项目(202213030509) （202213030509）
