山东科学2024，Vol.37Issue(5)：17-24,8.DOI:10.3976/j.issn.1002-4026.20230176
吲哚乙酰化高直链玉米淀粉改善溃疡性结肠炎的作用机制研究
Mechanism of action indole acetylated high-amylose maize starch in improving ulcerative colitis
摘要
Abstract
The aim of this study is to investigate the mechanism action of indole acetylated high-amylose maize starch in improving ulcerative colitis.Dextran sulfate sodium salt was used to induce acute ulcerative colitis in mice.Indole acetylated high-amylose maize starch was then used to target the colon with the delivery of indole-3-acetic acid.This was combined with the administration of an aryl hydrocarbon receptor inhibitor to analyze the mice in terms of colon length;disease activity index;and levels of interleukin-6,interleukin-10,transforming growth factor β1,myeloperoxidase,and interleukin-22 in the colon,which were detected by enzyme-linked immunosorbent assay.Quantitative polymerase chain reaction was used to analyze the relative expression levels of the colonic tight junction proteins occludin,ZO-1,and CYP1A1.Flow cytometry was used to test the ratio of regulatory T cells and T helper 17 cells in the mesenteric lymph nodes.The results showed that administration of indole acetylated high-amylose maize starch significantly alleviated the shortening of the colon length;significantly reduced the disease activity index and the levels of interleukin-6 and myeloperoxidase;significantly promoted the expression of interleukin-10,transforming growth factor β1,interleukin-22,CYP1A1,occludin,and ZO-1;significantly increased the proportion of regulatory T cells;and significantly reduced the proportion of T helper 17 cells.Administration of the aryl hydrocarbon receptor inhibitor weakened the effect of indole acetylated high-amylose maize starch.These results together suggest that acetylated high-amylose maize starch can improve ulcerative colitis by activating aryl hydrocarbon receptors.关键词
肠道菌群代谢产物/吲哚乙酸/溃疡性结肠炎/芳香烃受体/炎症反应/免疫平衡/色氨酸Key words
microbiota metabolites/indole-3-acetic acid/ulcerative colitis/aryl hydrocarbon receptors/inflammatory response/immune balance/tryptophan分类
药学引用本文复制引用
曲新艳,李庆军,丁兴春,宋营营..吲哚乙酰化高直链玉米淀粉改善溃疡性结肠炎的作用机制研究[J].山东科学,2024,37(5):17-24,8.基金项目
国家自然科学基金项目(82100555,82304430) （82100555,82304430）
科教产融合试点工程基础研究类项目(2022PY068) （2022PY068）
校(院)人才科研项目(2023RCKY085) （院）
