山东科学2024，Vol.37Issue(5)：25-34,10.DOI:10.3976/j.issn.1002-4026.20240015
新加苁蓉菟丝汤通过线粒体自噬途径促卵泡发育的分子机制
The molecular mechanisms by which Xinjia Cistanche Tusi Decoction promotes follicular development through mitophagy
摘要
Abstract
Using light chromatography-mass spectrometry(LC-MS),network pharmacology,and animal experiments,we explored the molecular mechanism by which Xinjia Cistanche Tusi Decoction(XJCRTST)promotes follicular development through granulosa cell mitophagy.LC-MS and network pharmacology were used to screen for the main components of XJCRTST and its therapeutic targets and pathways related to hypoovarianism.Female Sprague-Dawley rats were divided into five groups:model group,western medicine group,and XJCRTST high dose,medium dose,and low dose groups.The morphological changes of the ovary were observed through hematoxylin and eosin staining,and the structure and morphology of mitochondria and mitophagosomes in ovarian granulosa cells were observed through transmission electron microscopy.Two hundred and fifty-two compounds were identified in XJCRTST,with 42 potential targets for treating hypoovarianism,and the Gene Ontology enrichment mainly involved biological process(1 168 items),cell composition(45 items),and molecular function(59 items).KEGG pathway enrichment showed that the pathways involved were AGE-RAGE,Prolactin and TNF signaling pathways.Three target genes Parkin/PINK1,HIF1A and MAPK8 were enriched in the Mitophagy-animal process.Animal experiments showed that the number of follicles increased and the atretic follicles decreased in the Chinese medicine treatment group.Electron microscopy found that mitochondrial swelling and mitophagy decreased in the Chinese medicine group.XJCRTST may play a therapeutic role in treating hypoovarianism by regulating mitochondrial function and mitophagy-related pathways in ovarian granulosa cells.关键词
新加苁蓉菟丝汤/卵巢功能减退/网络药理学/液相色谱-质谱联用仪/线粒体Key words
Xinjia Cistanche Tusi Decoction/ovarian hypoplasia/network pharmacology/LC-MS/mitochondria分类
中医学引用本文复制引用
严谨,李翡,吴克明,杨皓博..新加苁蓉菟丝汤通过线粒体自噬途径促卵泡发育的分子机制[J].山东科学,2024,37(5):25-34,10.基金项目
2024年度陕西省科技厅项目一般项目(青年)(2024JC-YBQN-0892) （青年）
2021年陕西省教育厅科学研究项目(21JS017) （21JS017）
2021年度陕西中医药大学校级科研课题 （）
