二穗短柄草TCP转录因子的全基因组鉴定及其对非生物胁迫的响应OA北大核心CSTPCD

Brachypodium distachyon is an annual temperate Pooideae plant with abundant genetic resources and close bloodline relationship with cereal crops.It is an important cereal model plant.TEOSINTE BRANCHED1/CINCINNATA/PROLIFERATING CEL FACTOR(TCP)family is plant-specific transcription factor that widely regulates plant growth and development,as well as respond to abiotic stress.However,information about Brachypodium distachyon TCP transcription factors remain unclear.Here,a genome-wide identification of TCP genes was performed in Brachypodium distachyon,and 21 TCP genes(BdTCPs)were identified.The classification results showed that 21 BdTCPs were mainly divided into two clades as follows:11 PROLIFERATING CEL FACTOR(PCF)genes(Class Ⅰ),7 CINCINNATA(CIN)genes and 3 CYCLOIDEA(CYC)genes(Class Ⅱ).Sequence analysis showed that BdTCP proteins all contain TCP/TCP superfamily domain,and have different exon-intron structures.Motif analysis showed that members of the BdTCP family all contain Motif 1 and Motif 2,indicating that these two motifs are conserved regions necessary for their fuctions.Cis-acting element analysis indicated that the promoter regions of all BdTCPs contained phytohormone and environmental response elements,suggesting that BdTCPs may be involved in abiotic stress response.Quantitative PCR results showed 21 BdTCPs were insensitive to salt stress,but two of them(BdTCP10 and BdTCP15)were involved in the transient response to drought stress,and their expression levels were significantly up-regulated,indicating that BdTCP10 and BdTCP15 genes play a positive regulatory role in the response of Brachypodium distachyon to drought stress.The above results provide important clues for further research on the role of TCP in regulating the growth and development of Brachypodium distachyon and its abiotic stress response.