山东农业大学学报（自然科学版）2024，Vol.55Issue(5)：694-701,8.DOI:10.3969/j.issn.1000-2324.2024.05.007
厚皮甜瓜'SN-1'组培再生和遗传转化体系的建立
Establishment of Tissue Culture and Genetic Transformation Systems for Thick-peeled Melon'SN-1'
摘要
Abstract
We used the self-pollinated line'SN-1'(Cucumis melo ssp.melo)of muskmelon as the experimental material.By screening conditions for seed and explant treatment,culture medium components,hormone ratios,and co-culture conditions,a tissue culture regeneration and genetic transformation system for the self-pollinated line'SN-1'of muskmelon was established.The results indicated that the germination time of seeds on Agar medium was significantly better than on 1/2MS+Agar and MS+Agar;analyzing the effects of different concentrations(2%,3%,and 4%,v/v)of NaClO disinfectant and treatment times(10,15,20,and 25 min)on seed germination revealed that treatment with 3%NaClO for 15 minutes resulted in significantly higher germination rates and uniformity compared to other treatments;compared to the"binary method,"the"quaternary method"can obtain more cotyledonary nodes without affecting differentiation,significantly improving seed utilization;further analysis found that collecting cotyledonary nodes from seeds with a radicle length of about 5 mm at 48 hours after sowing resulted in significantly better adventitious bud differentiation than other periods;research on the effects of different hormone ratios and concentrations on cotyledonary nodes revealed that the best adventitious bud differentiation was achieved with concentrations of 0.5 mg·L-1 6-BA,0.05 mg·L-1 IAA,and 1 mg·L-1 ABA in the culture medium;when adding 0.4 mg·L-1 IAA to the rooting medium,the induction effect of adventitious roots in the regenerated seedlings was significantly better than other treatments;analysis of the co-culture period and medium hormone ratios after infection revealed that the best cotyledonary node development occurred with a 3-day co-culture period and the addition of 0.5 mg·L-1 6-BA+0.1 mg·L-1 IAA to the medium.关键词
厚皮甜瓜/组培再生/遗传转化Key words
Cucumis melo L.ssp.melo/tissue culture/genetic transformation分类
农业科技引用本文复制引用
赵欣冉,孙科新,王建全,胡宗霞,郭永杰,史庆华,杨晓玉..厚皮甜瓜'SN-1'组培再生和遗传转化体系的建立[J].山东农业大学学报（自然科学版）,2024,55(5):694-701,8.基金项目
国家自然科学基金面上项目(32272795) （32272795）
山东省泰山学者青年专家项目(tsqnz20230605) （tsqnz20230605）
山东省自然科学基金面上项目(ZR2022MC029) （ZR2022MC029）
山东省农业良种工程项目(2022LZGCQY006、2023LZGCQY021) （2022LZGCQY006、2023LZGCQY021）
山东省蔬菜产业技术体系项目(SDAIT-05) （SDAIT-05）
