山东农业大学学报（自然科学版）2024，Vol.55Issue(5)：733-739,7.DOI:10.3969/j.issn.1000-2324.2024.05.012
不同油茶品种间儿茶素含量差异及油茶炭疽病关系
Differences in Catechin Content Among Different Camellia Oleifera Varieties and Connection
摘要
Abstract
To explore the connection between the composition and content of catechin monomers in leaves of different Camellia oleifera varieties and leaf anthracnose disease,the content of four major catechin monomer compounds in the leaves of Changlin series C.oleifera varieties was measured.The differences between varieties,the distribution characteristics of leaf positions,and the correlation with anthracnose disease were analyzed to provide a basis for the selection of high-resistant C.oleifera varieties.The content of four main catechin compound monomers,including epicatechin(EC),epigallocatechin(EGC),epicatechin gallate(ECG),and epigallocatechin gallate(EGCG),in leaves from different parts of C.oleifera branches were determined by high-performance liquid chromatography(HPLC).We investigated the disease situation of different leaf positions on branches and analyzed the differences of catechin monomeric compounds among C.oleifera clones and their correlation with diseases.The results showed that there were differences in the average content of catechin monomers in different clones of C.oleifera leaves,but not significant(P>0.05);In C.oleifera leaves,the content of ester type catechin compounds(EGCG,ECG)exceeds 50%,among which epigallocatechin gallate ester(EGCG)has the highest content and significant differences from other catechins(P<0.05);The incidence of anthracnose in C.oleifera leaves shows a negative correlation with the total content of catechin monomer compounds along the spatial position of C.oleifera branches,with a correlation degree reaching moderate or above.The experiments demonstrate that the content of the four major catechin monomer compounds correlates with the quality and disease resistance of C.oleifera,and can serve as indicators for comprehensive evaluation in the selection of superior strains for quality and disease resistance in C.oleifera.The ester-type catechin compounds(EGCG,ECG)can provide a basis for preliminary rapid screening of superior strains.The specific universal applicability and the concentration of the four major catechin monomers in inhibiting pathogens on C.oleifera leaves,as well as their synergistic mechanisms,require further study.关键词
油茶/儿茶素/抗病性Key words
Camellia oleifera/catechin/disease resistance/quality分类
农业科技引用本文复制引用
陈伟,顿春垚,李双龙,丁中彪,姚小华,吴代坤..不同油茶品种间儿茶素含量差异及油茶炭疽病关系[J].山东农业大学学报（自然科学版）,2024,55(5):733-739,7.基金项目
国家重点研发计划项目课题"酉阳县油茶产业绿色高效生产技术集成示范"(2022YFD1601402) （2022YFD1601402）
中央财政林业科技推广示范项目"恩施州油茶低产林改造技术推广示范"(鄂[2023]TG09号) （鄂[2023]TG09号）
