山东农业大学学报（自然科学版）2024，Vol.55Issue(5)：774-781,8.DOI:10.3969/j.issn.1000-2324.2024.05.017
黄瓜FtsZs基因家族鉴定及分析
Identification and Analysis of FtsZs Family in Cucumber
摘要
Abstract
FtsZ protein,as a homolog of tubulin,often assembles into a contractile cytoskeletal element known as the"Z"ring,influencing plastid division.However,research on cucumber FtsZ proteins has not been reported,and its function is unclear.In this paper,we identified three FtsZ proteins in cucumber by bioinformatics methods and classified them into two groups.Sequence analysis revealed that their lengths range from 4 493 to 6 264 bp,containing 6 to 7 exons,with amino acid lengths of 421 to 488 AA,located on chromosome 3 or 6;subcellular localization predictions indicated that cucumber FtsZ is located in the chloroplast or cytoplasm;RNA-seq analysis found that cucumber FtsZs are highly expressed in stem,leaf,female flower,and ovary,and fluorescence quantitative PCR results showed that the expression level of cucumber CsFtsZ2-1 gene is also high in male flowers,indicating that cucumber FtsZs genes play a role in the growth and development of leaves,flowers,and ovaries;additionally,in the process of responding to coercion,the CsFtsZ2-1 gene can respond to salt stress,CsFtsZ1,CsFtsZ2-1,and CsFtsZ2-2 can respond to cold stress,and CsFtsZ1 and CsFtsZ2-1 can respond to powdery mildew pathogen infection.The research results provide a reference and basis for further study of the role and mechanism of FtsZs genes in cucumber.关键词
黄瓜/FtsZs基因/生物信息学分析/非生物胁迫反应/生物胁迫反应Key words
Cucumber/FtsZ gene/bioinformatics analysis/abiotic stress response/biotic stress response分类
农业科技引用本文复制引用
陈琳杰,常婧姝,张宇,颜爽,任仲海,王丽娜..黄瓜FtsZs基因家族鉴定及分析[J].山东农业大学学报（自然科学版）,2024,55(5):774-781,8.基金项目
国家自然科学基金(31972419 （）
31672170) （）
山东"双一流"专项建设基金(SYL2017YSTD06) （SYL2017YSTD06）
山东省泰山学者建设工程(ts20130932) （ts20130932）
山东省重点研发计划(农业良种工程)项目(2022LZGCQY001) （农业良种工程）
