针灸治疗浆细胞性乳腺炎的疗效观察及对JAK2/STAT3信号通路关键蛋白的影响OACSTPCD
Efficacy observation of acupuncture-moxibustion in treating plasma cell mastitis and the effects on key proteins of JAK2/STAT3 signaling pathway
目的 观察针灸治疗浆细胞性乳腺炎的临床疗效及对非受体型酪氨酸蛋白激酶 2(Janus kinase 2,JAK2)/信号转导及转录激活蛋白3(signal transducer and activator of transcription 3,STAT3)信号通路关键蛋白的影响.方法 将96 例浆细胞性乳腺炎患者随机分为观察组和对照组,每组48 例.对照组予甲泼尼龙,观察组在对照组基础上予针刺和艾灸治疗.比较两组临床疗效、复发情况及不良反应发生情况,观察两组治疗前后乳房症状(乳房肿块直径、乳房肿块范围、乳房疼痛和乳房皮肤)评分,血清炎性因子[肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)、白介素-8(interleukin-8,IL-8)和 C 反应蛋白(C-reactive protein,CRP)]、血清免疫指标[免疫球蛋白 G(immunoglobulin G,IgG)、免疫球蛋白M(immunoglobulin M,IgM)、补体C3和C4]以及乳房组织液中JAK2/STAT3信号通路关键蛋白[JAK2、磷酸化JAK2(phospho-JAK2,p-JAK2)和STAT3、磷酸化STAT3(phospho-STAT3,p-STAT3)]的水平.结果 观察组总有效率为 97.9%,高于对照组的 75.6%(P＜0.05);治疗后 3 个月随访时,观察组复发率 2.2%,低于对照组的 26.5%(P＜0.05).治疗后,观察组乳房肿块直径、乳房肿块范围、乳房疼痛和乳房皮肤评分均低于对照组(P＜0.05),观察组TNF-α、IL-6、IL-8、CRP、IgG、IgM、补体C3、补体C4、JAK2、p-JAK2、STAT3和p-STAT3 水平均低于对照组(P＜0.05).对照组不良反应发生率为 31.1%,高于观察组的 23.4%(P＜0.05).结论 针灸联合甲泼尼龙治疗浆细胞性乳腺炎的临床疗效优于单纯甲泼尼龙治疗,可更好地缓解乳房肿块、疼痛等症状,降低复发,其作用机制可能与调节JAK2/STAT3 信号通路关键蛋白有关.
Objective To observe the clinical efficacy of acupuncture-moxibustion in treating plasma cell mastitis and the effects on key proteins of the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Method Ninety-six patients with plasma cell mastitis were randomized into an observation group and a control group,each consisting of 48 cases.The control group was given Methylprednisolone,and the observation group received additional acupuncture and moxibustion treatment based on the intervention for the control group.The clinical efficacy,recurrence,and adverse reactions were compared between the two groups.Before and after the treatment,both groups were observed for breast symptom scores(breast lump diameter,breast lump extent,breast pain,and the skin of the breast),serum inflammatory factor levels[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-8(IL-8),and C-reactive protein(CRP)],serum immune indexes[immunoglobulin G(IgG),immunoglobulin M(IgM),complement 3(C3),and complement 4(C4)],and the levels of key protein levels of the JAK2/STAT3 signaling pathway in the breast tissue fluid[JAK2,phosphorylated JAK2(p-JAK2),STAT3,and phosphorylated STAT3(p-STAT3).Result The total effective rate was 97.9%in the observation group,higher than 75.6%in the control group(P＜0.05);at the 3-month post-treatment follow-up,the relapse rate was 2.2%in the observation group,lower than 26.5%in the control group(P＜0.05).After the treatment,the breast lump diameter,breast lump extent,breast pain,and breast skin scores were lower in the observation group than in the control group(P＜0.05),and the levels of TNF-α,IL-6,IL-8,CRP,IgG,IgM,C3,C4,JAK2,p-JAK2,STAT3,and p-STAT3 in the observation group were lower than those in the control group(P＜0.05).The adverse reaction rate was 31.1%in the control group,higher than 23.4%in the observation group(P＜0.05).Conclusion Acupuncture-moxibustion plus Methylprednisolone can produce more significant clinical efficacy than using Methylprednisolone alone in treating plasma cell mastitis.This combined method can better relieve symptoms such as breast lump and pain and lower recurrence,and the mechanism may be related to regulating key proteins of the JAK2/STAT3 signaling pathway.
赵莉;栗粟
河南省中医院,郑州 450002
中医学
针灸疗法艾条灸乳腺炎女性非受体型酪氨酸蛋白激酶2信号转导及转录激活蛋白3
Acupuncture-moxibustionMoxa stick moxibustionMastitisFemaleJanus kinase 2Signal transducer and activator of transcription 3
《上海针灸杂志》 2024 (010)
1091-1097 / 7
河南省中医药科学研究专项课题(2022ZY1081)
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