上海针灸杂志2024，Vol.43Issue(10)：1139-1146,8.DOI:10.13460/j.issn.1005-0957.2024.13.4006
针刺通过良性调控反应性星形胶质细胞极化改善后循环缺血性眩晕
Acupuncture improves posterior circulation ischemic vertigo via positive regulation of reactive astrocyte polarization
摘要
Abstract
Objective To observe the effect of acupuncture on reactive astrocyte polarization in the hippocampus of rats with posterior circulation ischemic vertigo(PCIV).Method Thirty-two clean-grade male Sprague-Dawley(SD)rats were randomized into a sham operation group,a model group,a conventional acupuncture group,and a cervical transverse process tip acupuncture group,each consisting of 8 rats.The PCIV model was developed by surgical ligation of the right common carotid artery and subclavian artery.The conventional acupuncture group was intervened by acupuncture at Baihui(GV20)and bilateral Fengchi(GB20)and Neiguan(PC6).The cervical transverse process tip acupuncture group received acupuncture at the cervical transverse process tips of C2,C4,and C6.The Sham operation group and model group only underwent grasping and fixing.The interventions were all conducted once daily over 14 d.After the intervention,laser Doppler flowmetry was used to detect the blood flow in the rat's vestibular nerve nucleus(VNN);the Western blot(WB)assay was adopted to determine the expression levels of reactive astrocyte marker glial fibrillary acidic protein(GFAP),A1 astrocyte marker C3d,and A2 astrocyte marker S100 calcium binding protein A10(S100A10)in the hippocampus of rats.Changes in the numbers of hippocampal A1 and A2 astrocytes were observed using immunofluorescence double staining.Result Compared to the sham operation group,the model group rats showed decreased VNN blood flow(P＜0.01),an increased balance beam test score(P＜0.01),elevated C3d expression and dropped S100A10 expression in the hippocampus(P＜0.01),and a larger number of hippocampal A1 astrocytes and a smaller number of A2 astrocytes(P＜0.01).Compared to the model group,rats in the two acupuncture groups presented elevated VNN blood flow(P＜0.01),a decreased balance beam test score(P＜0.01),lower C3d expression and higher S100A10 expression in the hippocampus(P＜0.05),and a smaller number of A1 cells and a larger number of A2 cells(P＜0.01).Compared to the conventional acupuncture group,rats in the cervical spinal process tip acupuncture group had elevated VNN blood flow(P＜0.01),a decreased balance beam test score(P＜0.01),lower C3d expression and higher S100A10 expression,and a smaller number of A1 cells and a larger number of A2 cells,while the latter had no statistical significance(P＞0.05).Conclusion Acupuncture can raise vestibular blood flow,inhibit the expression of nociceptive A1 astrocytes,and enhance the expression of protective A2 astrocytes in PCIV rats.The mechanism of acupuncture treating PCIV may be associated with the positive control of reactive astrocyte polarization.关键词
针刺疗法/电针/后循环缺血性眩晕/星形胶质细胞极化/大鼠Key words
Acupuncture therapy/Electroacupuncture/Posterior circulation ischemic vertigo/Astrocyte polarization/Rats分类
医药卫生引用本文复制引用
文林月,杨丽芳,欧小燕,柳刚..针刺通过良性调控反应性星形胶质细胞极化改善后循环缺血性眩晕[J].上海针灸杂志,2024,43(10):1139-1146,8.基金项目
安徽省高校自然科学研究项目(2023AH050760) （2023AH050760）
安徽省重大疑难疾病中西医协同攻关项目计划[皖中医药发展秘(2021)70号] （2021）
