两种国产膀胱癌荧光原位杂交检测试剂盒的临床应用比对OACSTPCD
Comparative clinical application of two domestic fluorescence in situ hybridization kits in bladder cancer
目的:通过比对两种国产膀胱癌荧光原位杂交(Fluorescence In Situ Hybridization,FISH)试剂盒在尿液脱落细胞染色体畸变检测结果差异,探究膀胱癌FISH试剂盒的实际临床应用价值.方法:选取 2021年 1月至 2022年1月,首都医科大学临床检验中心 20例血尿患者尿液筛查样本,分别使用不同厂家试剂盒,检测尿液脱落细胞染色体畸变,并与液基薄层细胞特殊染色(CellDetect染色)结果进行对比,分析两种不同试剂盒检测结果的差异.结果:20 例样本中,19 例样本检测结果一致,仅有 1 例样本结果不一致.两种试剂盒阳性检出率分别为 50%和 45%,结果无统计学差异(P>0.05).结论:两种国产膀胱癌FISH试剂盒检测结果一致性高,均可应用于临床膀胱癌FISH检测.
Objective:Comparing the detection results of malignant urothelial cells isolated from voided urine between two domestic Fluorescence In Situ Hybridization(FISH)kits and probe the potential clinical value of FISH.Methods:Urine of 20 patients were collected from Center for Clinical Laboratory,Capital Medical University between January 2021 to January 2022.We used different domestic kits to test the different results of chromosomal aberrations and then compared with CellDetect® staining.Results:Only one patient with the test results of two domestic kits were inconsistent.And the results show that the remaining patients were consistent with the assay result(P>0.05).The positive detection rates of the two kits were 50%and 45%,respectively.Conclusion:We found that the high concordance of the results of chromosomal aberrations with two different FISH kits and both kits can be detected bladder cancer in clinical laboratories.
张家淮;高朋;陈佳静;颜乐;赵炜;史秦峰
首都医科大学临床检验中心,北京 100069首都医科大学临床检验中心,北京 100069首都医科大学临床检验中心,北京 100069首都医科大学临床检验中心,北京 100069首都医科大学临床检验中心,北京 100069首都医科大学基础医学院病理学系,北京 100069
膀胱癌荧光原位杂交CellDetect染色
bladder cancerfluorescence in situ hybridizationCellDetect staining
《现代科学仪器》 2024 (5)
40-45,6
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