现代科学仪器2024，Vol.41Issue(5)：51-54,4.
经长春海关关区输入的境外新型冠状病毒基因组特征分析
Analysis of genomic characteristics of SARS-CoV-2 imported from abroad through Changchun Customs
摘要
Abstract
To understand the demographic characteristics of imported patients with Coronavirus Disease 2019(COVID-19)and the whole genome variation of SARS-CoV-2 through Changchun Customs.The case information of 27 patients with COVID-19 imported through Changchun Customsfrom April to December 2023 was collected.The RT-PCR method was used to detect the nucleic acid of respiratory tract samples.The Ct values of ORF1ab gene and N gene were between 25 and 30,accounting for 55.56%.The whole genome of the virus was analyzed by the third-generation nanopore gene sequencer sequencing and the whole genome analysis V1.0 software,and the sequencing coverage was 98.02%-99.59%.Compared with the Wuhan reference strain(NC_045512.2),the number of nucleotide mutation sites in the SARS-CoV-2 genome sequence was between 62 and 132.Pangolin typing showed that all of them were Omicron mutant strains,EG.5.1 and its sub-branches accounted for the largest proportion,accounting for 25.93%.By analyzing the nucleic acid and gene sequence of the novel coronavirus of entry personnel at the ports through Changchun Customs and monitoring the import of SARS-CoV-2 abroad,the variation trend of the virus strain can be grasped in real time.关键词
新型冠状病毒/Omicron变异株/境外输入/全基因组三代测序Key words
SARS-CoV-2/Omicron variant strain/Imported cases/Whole genome third-generation sequencing引用本文复制引用
丁旭,张井巍,王玮琳,马文晨,刘韬,石洋,孙丽华,张婷..经长春海关关区输入的境外新型冠状病毒基因组特征分析[J].现代科学仪器,2024,41(5):51-54,4.基金项目
海关总署科研项目(2021HK190). （2021HK190）
