CYP2E1稳定高表达肝细胞系的构建及其在药物性肝损伤活性筛选模型中的应用OACSTPCD

Drug-related liver injury is one of the most common and serious adverse drug reactions in clinic.Acet-aminophen,a commonly used clinical antipyretic and analgesic,is a common factor causing drug-related liver in-jury.The hepatic microsomal enzyme cytochrome P450 2E1(CYP2E1)plays an important role in acetaminophen-induced hepatocyte injury.Since in vitro cultured hepatocyte lines barely express microsomal enzyme CYP2E1,a hepatocyte line stably and highly expressing CYP2E1 gene were conducted using the human hepatoma cell line HepG2 transfected by CYP2E1-lentivirus,and an assay for screening inhibitors for acetaminophen-induced hep-atocyte injury was performed with this cell line.The results showed that eight natural compounds with potential in vitro activity against drug-induced liver injury were identified.This study provides a suitable in vitro screening model for drug-induced liver injury,and the results from the screening also provide a theoretical basis for the re-search and development of the traditional Chinese medicine Cyperus rotundus L..