胶质母细胞瘤耐药性基因筛选及治疗药物预测OA

Objective To screen differentially expressed genes(DEGs)of temozolomide(TMZ)resistance in glioblastoma cells(GBM)by bioinformatics technology,explore the key drug resistance genes and pathogenesis,and predict the related traditional Chinese medicine.Methods Based on GBM,GEO2R,GPEIA and other databases,biological function and pathway enrichment analysis were carried out,and protein interaction network was constructed to screen key genes.The effects of key genes on mRNA expression and survival rate in normal human and glioma patients were searched.The COREMINE platform was further used to construct a drug-active ingredient-target network for key drug resistance genes,and the active ingredients of related Chinese medicines were screened.Results A total of 293 DEGs were screened,involving key targets such as FN1,CD44,CTGF,and FOS.The expression was significantly higher than that of normal people,affecting the overall survival rate of patients,and was a harmful prognostic factor for GBM,but it did not affect the recurrence-free survival rate.While it affected biological functions such as cell adhesion,nervous system development,transforming growth factor β receptor signal transduction and gene positive regulation.It was predicted by COREMINE that the intervention drugs closely related to FN1 were mostly attributed to the liver meridian,and the nature and flavor were mainly heat-clearing and deficiency-tonifying.Conclusion Twelve key genes related to TMZ resistance in GBM were screened out.FN1 plays an important role in the occurrence and development of TMZ resistance.The active components of Muxiang,Qingyedan and Bimazi may be used as the source of TMZ resistance drugs.