中国当代儿科杂志2024，Vol.26Issue(10)：1115-1121,7.DOI:10.7499/j.issn.1008-8830.2407088
普萘洛尔对人脐静脉内皮细胞生物学行为及SOX18、MMP-7、VEGFA表达的影响
Effects of propranolol on the biological behavior of human umbilical vein endothelial cells and the expression of SOX18,MMP-7,and VEGFA
摘要
Abstract
Objective To investigate the effects of propranolol on the proliferation,apoptosis,migration,and tube formation ability of human umbilical vein endothelial cells(HUVEC),as well as its impact on the expression of sex-determining region Y-box 18(SOX18),matrix metalloproteinase-7(MMP-7),and vascular endothelial growth factor A(VEGFA).Methods HUVEC were treated with different concentrations of propranolol,and cell viability was assessed using the CCK-8 method to determine the optimal concentration and treatment duration.The experiment consisted of a control group and groups treated with different concentrations of propranolol(50,100,150 μmol/L).Apoptosis,migration,and tube formation of HUVEC were observed using flow cytometry,wound healing assays,and tube formation assays.Western blot and real-time quantitative PCR were used to detect the expression levels of SOX18,MMP-7,and VEGFA proteins and mRNA.Results Compared to the control group,the apoptosis rate in the propranolol treatment groups increased significantly(P<0.05),and it rose significantly with increasing drug concentration(P<0.05).The wound healing rate decreased in the propranolol treatment groups,and both the number of tube formation nodes and total tube length were reduced(P<0.05).The expression levels of SOX18,MMP-7,and VEGFA proteins and mRNA were downregulated in the propranolol treatment groups(P<0.05).Conclusions Propranolol can inhibit the proliferation,migration,and tube formation ability of HUVEC and promote cell apoptosis,resulting in decreased expression levels of SOX18,MMP-7,and VEGFA.关键词
普萘洛尔/婴幼儿血管瘤/性别决定区Y框18/基质金属蛋白酶-7/血管内皮生长因子A/人脐静脉内皮细胞Key words
Propranolol/Infantile hemangioma/Sex-determining region Y-box 18/Matrix metalloproteinase-7/Vascular endothelial growth factor A/Human umbilical vein endothelial cell引用本文复制引用
周佩,谢思琴,钟礼立,丁小芳..普萘洛尔对人脐静脉内皮细胞生物学行为及SOX18、MMP-7、VEGFA表达的影响[J].中国当代儿科杂志,2024,26(10):1115-1121,7.基金项目
湖南省卫生健康委一般资助课题基金(202106011804). （202106011804）
