中国人兽共患病学报2024，Vol.40Issue(9)：823-828,6.DOI:10.3969/j.issn.1002-2694.2024.00.122
鸭源A型鹦鹉热衣原体的分离鉴定
Isolation and identification of genotype A Chlamydia psittaci strain from duck
摘要
Abstract
Chlamydia psittaci is a worldwide distributed zoonotic pathogen that infects a variety of birds.In order to char-acterization of the duck originated C.psittaci strains,lung samples were collected from suspected infection ducks and was de-tected by real-time PCR.The positive samples were homogenized in phosphate buffered saline with kanamycin and streptomy-cin,and then inoculated onto L929 cells monolayer.After several sets of passages,chlamydial inclusions of the isolate in cul-tured cells were observed after Giemsa staining or by electron microscope.For species identification,16S rRNA,16-23S IGS gene fragments were sequenced and analyzed.Genotyping of the isolate was performed by comparative analysis of the obtained ompA gene sequence with that of different genotype of C.psittaci strains.A C.psittaci strain was successfully isolated from the lung sample of duck by cell culture and was identified as genotype A.This study expanded our understanding of the host range of genotype A C.psittaci strain,and provided basis for further research on the pathogenicity,transmission,and public health risk of this pathogen.关键词
鹦鹉热衣原体/细胞培养/基因分型/鸭Key words
Chlamydia psittaci/cell culture/genoty-ping/duck分类
医药卫生引用本文复制引用
李兆才,刘萍,李赟辉,张淮瑜,肖乾,刘春国,周继章..鸭源A型鹦鹉热衣原体的分离鉴定[J].中国人兽共患病学报,2024,40(9):823-828,6.基金项目
国家重点研发计划项目(No.2022YFC2304001),中国农业科学院科技创新工程项目(No.CAAS-ASTIP-2021-LVRI) Supported by the National Key Research and Development Program of China(No.2022 YFC2304001)and the Innovation Program of Chinese Academy of Agricultural Sciences(No.CAAS-ASTIP-2021-LVRI). （No.2022YFC2304001）
