中国人兽共患病学报2024，Vol.40Issue(9)：829-833,840,6.DOI:10.3969/j.issn.1002-2694.2024.00.128
结肠小袋纤毛虫PCR-RFLP分型方法的建立
Establishment of a PCR-RFLP typing method for Balantioides coli
摘要
Abstract
This study established an efficient and specific method for type analysis of genetic variants of Balantioides coli.The restriction endonucleases ApoI and PflMI were selected to digest the PCR amplification products of ITS1-5.8S rDNA-ITS2 of B.coli,and to establish a PCR-RFLP typing method.The PCR-RFLP method was subsequently used to analyze the genetic variants in clinical fecal samples from pigs,sheep,and guinea pigs.The PCR-RFLP method based on ApoI and PflMI accurately distinguished the main A and B genetic variants of B.coli,and further divided the main B type into B-c and B-t sub-types of genetic variants with PflMI.Compared with the results of microscopy and sequencing,the PCR-RFLP method showed good specificity and higher sensitivity,and was able to identify not only single but also multiple variants of B.coli in a single clinical sample.This study successfully established the PCR-RFLP method for B.coli,which can be used for genetic di-versity identification and molecular epidemiological studies of B.coli.关键词
结肠小袋纤毛虫/PCR-RFLP分析/猪/羊/豚鼠Key words
Balantioides coli/PCR-RFLP analysis/pig/sheep/guinea pig分类
医药卫生引用本文复制引用
徐啊慧,冯彩彩,丰山旺,赵立卓,齐闻新,张雯,胡苏辉,王天奇,闫文朝..结肠小袋纤毛虫PCR-RFLP分型方法的建立[J].中国人兽共患病学报,2024,40(9):829-833,840,6.基金项目
Supported by the National Natural Science Foundation of China(No.31772733) 国家自然科学基金项目(No.31772733) （No.31772733）
