中国人兽共患病学报2024，Vol.40Issue(9)：880-886,7.DOI:10.3969/j.issn.1002-2694.2024.00.131
青海高原地区小型兽类7种病原体携带情况调查研究
Prevalence of seven zoonotic pathogens in small mammals in the Qinghai plateau region
摘要
Abstract
This study investigated the prevalence of Borrelia burgdorferi,Anaplasma phagocy tophilum,Rickettsia typhi,Orientia tsutsugamushi,Leptospira interrogans,Francisella tularensis,and Babesia spp.in small mammals in the Qinghai plateau region,to provide a scientific basis for the prevention and control of local zoonotic diseases.Small mammals were cap-tured with snap traps at six sampling sites in the Qinghai plateau region.Liver,spleen,and kidney tissues were collected for detection of six bacterial pathogens with real-time PCR.Conventional PCR(cPCR)was used for Babesia detection,and the positive PCR products were sequenced and analyzed.The differences in pathogen detection rates among species and habitats were analyzed with x2 test or Fisher's exact test.In to-tal,235 small mammals from 15 species were captured.B.burgdorferi,L.interrogans,and Babesia were detected in 11 spe-cies of small mammals,whereas A.phagocytophilum,R.typhi,O.tsutsugamushi,and F.tularensis were not detected.B.burgdorferi was detected in 41 small mammals from nine species(Cricetulus longicaudatus,Apodemus peninsulae,Ochotona curzoniae,Mus m usc ulus,Meriones meridians,Microtus arvalis,Cricetidae,Ochotona cansus,and Allactaga sibirica),with an infection rate of 17.45％(41/235).L.interrogans was detected in eight small mammals from four species(C.longicaudatus,M.musculus,M.arvalis,and Microtus oeconomus),with an infection rate of 3.40％(8/235).Babesia was detected in only one Mustela altaica,with an infection rate of 0.85％(1/235).Statistically significant differences were ob-served in the detection rates of pathogens among small mammal species(x2=200.54,P＜0.05).Among habitats,the detection rate of B.burgdorferi was highest in the forest(Fisher's exact test,P＜0.05).B.burgdorferi and L.interrogans co-infection was observed in three M.arvalis and two C.longicaudatus.In addition,one Babesia sequence was obtained,which clustered with Babesia vulpes in the phylogenetic tree.B.burgdorferi,L.interrogans,and Babesia were the main pathogens prevalent in small mammals in the Qinghai plateau region and have potential to cause human diseases.Local authori-ties should strengthen the surveillance of corresponding zoonotic diseases,and formulate corresponding prevention and control measures.关键词
人兽共患病/小型兽类/流行情况/青海高原地区Key words
zoonotic disease/small mammals/prevalence/Qinghai plateau region分类
医药卫生引用本文复制引用
程红兵,刘益萍,崔佳,饶华祥,栗冬梅,于娟..青海高原地区小型兽类7种病原体携带情况调查研究[J].中国人兽共患病学报,2024,40(9):880-886,7.基金项目
山西省基础研究计划项目(No.20210302124299,No.202303021221180) （No.20210302124299,No.202303021221180）
国家科技重大专项(No.2017ZX10303404) （No.2017ZX10303404）
山西省卫生健康委员会科研课题(No.2022119)和长治医学院学术技术带头人项目(No.XS202103)联合资助 Supported by the Natural Science Foundation of Shanxi Province of China(No.20210302124299,No.202303021221180),the National Science and Technology Major Project of China(No.2017ZX10303404),the Research Project of Shanxi Provincial Health Commission(No.2022119),and the Academic Technology Leader Project of Changzhi Medical College(No.XS202103) （No.2022119）
