中国兽医科学2024，Vol.54Issue(10)：1279-1286,8.DOI:10.16656/j.issn.1673-4696.2024.0156
O型口蹄疫病毒CATHAY株持续感染BHK21细胞系的建立
Establishment of a BHK21 cell line persistently infected by the CATHAY strain of foot-and-mouth disease O virus
摘要
Abstract
To investigate the molecular mechanism of the persistent infection of foot-and-mouth disease virus(FMDV),this research established a persistently infected cell line with O-type FMDV(CATHAY strain)and BHK21 cells using ammonium chloride,ribavirin,immune positive serum,and lower titer of FMDV.The persistent infection cell lines were screened and verified by RT-PCR,nucleic acid sequencing,immunofluorescence assay(IFA),and Western-blotting.The result showed that the BHK21 cell lines with FMDV persistent infection have been established by infection of lower titer of FMDV,the cell lines with FMDV can be continuously passaged.FMDV VP1 gene can be detectable from the 5th to 20th generation cells by RT-PCR and the sequences were highly homologous to the parental.The FMDV VP1 protein can be detectable by IFA in persistently infected cells.However,the remaining methods fail to develop the cell model of FMDV persistent infection due to their obvious toxic effects on cells.Therefore,we believed that the chemical drugs used in this study may be not a suitable reagent to develop the cell model of FMDV persistent infection,and direct infection using lower titer of FMDV is a simple and feasible method.Collectively,we successfully established BHK21 cell lines with FMDV persistent infection,which will helpful to investigate the interaction between FMDV and the host and clarifying the mechanism of FMDV persistent infection.关键词
口蹄疫病毒/持续感染细胞系/BHK21/利巴韦林/氯化铵/免疫阳性血清Key words
foot-and-mouth disease virus/persistently infected cell line/BHK21/ribavirin/ammonium chloride/immunopositive serum分类
农业科技引用本文复制引用
彭德财,丁嘉烽,刘伟,高闪电,马云云,温升辉,侯茁,师铭咸,邵军军,常惠芸..O型口蹄疫病毒CATHAY株持续感染BHK21细胞系的建立[J].中国兽医科学,2024,54(10):1279-1286,8.基金项目
"十四五"国家重点研发计划项目(2021YFD1800300,2021YFD1800303) （2021YFD1800300,2021YFD1800303）
