中国兽医科学2024，Vol.54Issue(10)：1287-1294,8.DOI:10.16656/j.issn.1673-4696.2024.0163
Rho GTP酶和肌动蛋白细胞骨架对新城疫病毒在细胞间传播的影响
Effects of Rho GTPase and actin cytoskeleton on the intercellular spread of Newcastle disease virus
摘要
Abstract
In this study,in order to explore the effects of Rho GTPase and actin cytoskeleton on NDV-induced intercellular membrane fusion and viral replication and spread,specific inhibitors tar-get to Rho GTPase and actin cytoskeleton were firstly used to treat cells and evaluated their effects on replication of NDV and intercellular membrane fusion induced by NDV.The results showed that inhibitor treatment could significantly inhibit the replication titer of NDV in cells and the intercellular mem-brane fusion induced by this virus.Furthermore,the observation results of laser scanning confocal mi-croscope showed that the fusion protein(F)of NDV and the actin of the cells were co-localized.Finally,the recombinant NDV expressing green fluorescent protein was rescued by reverse genetic technique,and the co-infection culture experiment revealed that Rho GTPase and actin cytoskeleton were involved in the transmission and spread of NDV among cells.The results of this study provided information for deep understanding of the role of Rho GTPase and actin cytoskeleton in the life cycle of NDV infection.关键词
新城疫病毒/Rho GTP酶/肌动蛋白细胞骨架/细胞间传播Key words
Newcastle disease virus/Rho GTP enzyme/actin cytoskeleton/intercellular spread分类
农业科技引用本文复制引用
张智颖,孙军峰,师乾凯,赵冉,王芳芳,李慧昕,韩宗玺,刘胜旺..Rho GTP酶和肌动蛋白细胞骨架对新城疫病毒在细胞间传播的影响[J].中国兽医科学,2024,54(10):1287-1294,8.基金项目
黑龙江省自然科学基金团队项目(TD2021C001) （TD2021C001）
畜禽重大疫苗科技成果转化及产业化项目(CG21B008) （CG21B008）
蛋鸡产业技术体系岗位科学家项目(CARS-40) （CARS-40）
