中国兽医科学2024，Vol.54Issue(10)：1295-1301,7.DOI:10.16656/j.issn.1673-4696.2024.0170
豆状囊尾蚴小胞外囊泡可抑制家兔外周血淋巴细胞NLRP3活化
Small extracellular vesicles from Cysticercus pisiformis can down-regulate the activation of NLRP3 in peripheral blood lymphocytes
摘要
Abstract
The aim of the study is to explore the expression of inflammasome during Cysticercus pisiformis infection.The eggs in gravid proglottids of Taenia pisiformis were collected and then used to infect rabbits.Primary peripheral blood lymphocytes(PBLC)at different times post infection with C.pisiformis were isolated and inflammasome related genes such as NLRP3 and IL-1β were detected.Be-sides,excretory-secretory products(ESP)and small extracellular vesicles(sEV)collected from C.pisi-formis were used to treat PBLC and inflammasome related genes were detected using qPCR and Western-blot after 24h.The results showed that the expression of NLRP3,IL-1β,Caspase-1 and GSDMD were significant-ly up-regulated at 30 days and 60 days post infection of C.pisiformis,and were down-regulated at 90 days.The expression of NLRP3,IL-1β,Caspase-1and GSDMD were significantly up-regulated in PBLC treated with ESP of C.pisiformis.The expression of IL-1β was significantly up-regulated after treating with sEV,but the expression of NLRP3,Caspase-1,IL-18 and GSDMD were inhibited.These results demonstrated that ESP and small extracellular vesicles can regulate the activation of inflammasome during C.pisi-formis infection,which may obtain a long-term survival and persistent infection of parasites.关键词
豆状囊尾蚴/NLRP3炎症小体/分泌排泄产物/小胞外囊泡Key words
Cysticercus pisiformis/NLRP3 inflammasome/excretory-secretory products/small extra-cellular vesicles分类
农业科技引用本文复制引用
蒲桂婷,王立群,刘婷丽,王得先,曹珊菱,郭小腊,殷宏,骆学农..豆状囊尾蚴小胞外囊泡可抑制家兔外周血淋巴细胞NLRP3活化[J].中国兽医科学,2024,54(10):1295-1301,7.基金项目
国家自然科学基金项目(32072889) （32072889）
