中国兽医科学2024，Vol.54Issue(10)：1302-1308,7.DOI:10.16656/j.issn.1673-4696.2024.0171
猪流行性腹泻病毒IgA抗体ELISA检测方法的建立和初步应用
Establishment and preliminary application of an ELISA method for detection of porcine epidemic diarrhea virus IgA antibody
摘要
Abstract
To identify the IgA antibody against porcine epidemic diarrhea virus(PEDV),an ELISA technique was developed and refined using PEDV recombinant S protein as the coating antigen and horseradish peroxidase-conjugated murine anti-porcine IgA monoclonal antibody as the identifying an-tibody,and its specificity,sensitivity,reproducibility,and performance of clinical samples were evaluated.The findings indicated that the optimal antigen encapsulation mass concentration was 2 μg/mL,the best enzyme-labeled antibody working concentration was 1∶25 000,and the most effective serum di-lution was 1∶50.There was no cross-reactivity with positive serums of porcine pseudorabies virus,African swine fever virus,classical swine fever virus,Senecavirus A and foot-and-mouth disease virus.The sensitivity was determined to be 1∶800.The coefficients of variation were less than 10％within and among different batches,and the consistency rate with IDEXX commercial kit was very high in testing result.These results prove that the detection method for PEDV IgA antibody developed in this research has good specificity,sensitivity and reproducibility,making it suitable for clinical diagnosis,di-sease prevention,and control of porcine epidemic diarrhea,as well as for epidemiological studies and evaluation of vaccination efficacy.关键词
猪流行性腹泻病毒/S蛋白/IgA抗体/ELISAKey words
porcine epidemic diarrhea virus/S protein/IgA antibody/ELISA分类
农业科技引用本文复制引用
王婉莹,石正旺,罗俊聪,廖焕程,冯露,郑海学,田宏,蔺国珍..猪流行性腹泻病毒IgA抗体ELISA检测方法的建立和初步应用[J].中国兽医科学,2024,54(10):1302-1308,7.基金项目
甘肃省科技重大专项(22ZD6NA012,22ZD6NA001) （22ZD6NA012,22ZD6NA001）
甘肃省自然科学基金项目(21JR1RA203) （21JR1RA203）
中央高校基本科研业务费专项(lzujbky-2022-ct02) （lzujbky-2022-ct02）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
