中国兽医科学2024，Vol.54Issue(10)：1345-1350,6.DOI:10.16656/j.issn.1673-4696.2024.0176
ASFV和PRV野毒及PRV疫苗毒多重纳米PCR检测方法的建立及应用
Establishment and application of multiplex nano-PCR detection method for ASFV,PRV wild virus and PRV vaccine virus
摘要
Abstract
In order to establish a method for simultaneous detection of ASFV,PRV wild virus and PRV vaccine virus(gE-),a multiplex nano-PCR method for simultaneous detection of ASFV,PRV wild virus and PRV vaccine virus were established by using the B646L gene of ASFV and the gE and gD gene of PRV as the detection objects.The method's specificity and sensitivity were studied and the results showed that it could specifically amplify the target genes of ASFV,PRV wild virus and PRV vaccine strain,but the re-suits were negative when detecting Seneca virus,porcine haemagglutinating encephalomyelitis virus,porcine vesicular stomatitis virus and porcine circovirus type 2.The minimum detection limits of B646L gene,gD gene and gE gene were 4,7 and 8 copies/μL,respectively.In addition,the effectiveness of the multiplex nano-PCR detection method was compared with commonly used commercial kits for these viruses,and the results showed that the coincidence rate was 100％.In summary,the multiplex nano-PCR method established in this experiment has high specificity and sensitivity and could simultaneously detect ASFV,wild-type and vaccine PRV,which could be used for differential diagnosis and disease prevention and control of major diseases in pigs.关键词
猪伪狂犬病病毒/非洲猪瘟病毒/多重纳米PCR检测方法/冷链猪肉Key words
pseudorabies virus/African swine fever virus/multiplex nano-PCR detection method/cold chain pork分类
农业科技引用本文复制引用
赵越,兰云刚,姚来顺,涂忠忠,曹泽昭,王炳量,薛珺,王改丽,贺文琦,宋德光..ASFV和PRV野毒及PRV疫苗毒多重纳米PCR检测方法的建立及应用[J].中国兽医科学,2024,54(10):1345-1350,6.基金项目
吉林省科技发展计划项目(0220203046SF) （0220203046SF）
