中国兽医科学2024，Vol.54Issue(10)：1368-1376,9.DOI:10.16656/j.issn.1673-4696.2024.0181
盘羊源多杀性巴氏杆菌与溶血性曼氏杆菌的分离与鉴定
Isolation and identification of Pasteurella multocida and Mannerella hemolytica from argali
摘要
Abstract
To investigate and characterize the pathogenic bacteria responsible for the death of argali in a zoo in Gansu Province,the lung tissue was collected from one argali dying of respiratory disorders with cough,dyspnea and other clinical symptoms.Bacteria were isolated and cultured from the collected samples and identified by morphological observation as well as 16S rRNA gene sequencing.The isolated strains were further characterized by capsular serotype identification,virulence gene de-tection,drug sensitivity test and pathogenicity test in mice.The results showed that a highly pathogenic Pasteurella multocida strain of serotype D and Mannerella hemolytica strain of serotype A2 was isolated and identified from the lung tissue of the argali.The isolated strain of Pasteurella mul-tocida serotype D carries 7 virulence genes including exbB,exbD,fur,tonB,hgBA,plpB and oma87,while the isolated strain Mannerella hemolytica serotype A2 carries 10 virulence genes including irp,ompA,plpE,tbpA,tbpB,fhaC,lkt,lpsA,nanH and gcp.The two isolates exhibited high susceptibility to amikacin,tobramycin,erythromycin,and norfloxacin.In addition,both of the two isolates were fatal to mice when inoculated.These results suggested that Pasteurella multocida serotype D and Mannerella hemolytica serotype A2 are the probable pathogens responsible for the death of the zoo argali in Gansu.The results also provided basis to carrying out measurements for prevention and treatment of the bacte-ria caused respiratory diseases in argali in the zoo.关键词
盘羊/多杀性巴氏杆菌/溶血性曼氏杆菌/血清型/毒力基因Key words
argali/Pasteurella multocida/Mannerobacter hemolyticus/serotype/virulence gene分类
农业科技引用本文复制引用
高桂琴,蔡江,曹小安,刘永杰,谷涛,兰喜,尚佑军,刘萍,何继军..盘羊源多杀性巴氏杆菌与溶血性曼氏杆菌的分离与鉴定[J].中国兽医科学,2024,54(10):1368-1376,9.基金项目
国家重点研发计划项目(2021YFD1800504,2022YFD1602203,2022YFD1302101) （2021YFD1800504,2022YFD1602203,2022YFD1302101）
国家现代农业产业技术体系资助项目(CARS-39-04B) （CARS-39-04B）
白银市2023年度高层次人才(团队)引进项目(BYTIP2023-10) （团队）
甘肃省科技特派团专项(22CX8NA012) （22CX8NA012）
