中国兽医科学2024，Vol.54Issue(10)：1408-1416,9.DOI:10.16656/j.issn.1673-4696.2024.0160
酵母β-葡聚糖对玉米赤霉烯酮诱导小鼠睾丸细胞铁死亡的缓解作用
Alleviation of zearalenone-induced ferroptosis in mouse testicular cells by yeast beta-glucan
摘要
Abstract
To investigate the effects of zearalenone(ZEA)on the expression and localization of ferroptosis-related genes in testicular tissues of male mice and the mitigating effects of β-glucan on ZEA-induced ferroptosis in mouse testicular cells.Fifty male mice were randomly divided into con-trol group(gavaged with corn oil),ZEA group(LG:0.5 mg/kg,MG:5 mg/kg,HG:50 mg/kg)and β-glucan group(TG:50 mg/kg ZEA+50 mg/kgβ-glucan)for 10 days.Taipan blue staining,HE staining,immunohistochem-istry,Western-blot and qRT-PCR were used to detect changes in the structure of mouse spermatozoa and testes and the expression and localization of the ferroptosis genes GPX4,SLC7A11 and FTH1.Compared with the control group,the ZEA group showed increased malformed spermatozoa,disorganized spermato-genic cells in the seminiferous tubules,elevated Fe content(P＜0.01)and MDA content(P＜0.001),de-creased SOD content(P＜0.001),and decreased expression of GPX4,SLC7A11,and FTH1 in the testicular tissues(P＜0.05).GPX4 was expressed in the nucleus of spermatogonia,SLC7A11 was expressed in the nu-cleus of spermatogonia and spermatocytes,and FTH1 was expressed in the nucleus and cell membrane of spermatogonia.Compared with ZEA group,β-glucan group had fewer abnormal sperm and more orderly sper-matogenic cells,Fe content(P＜0.05),MDA content(P＜0.01)decreased,SOD content increased(P＜0.001),and the expression of GPX4,SLC7All,and FTH1 was elevated(P＜0.05).The results showed that ZEA could cause testicular cell iron death by decreasing the expression of GPX4,SLC7A11 and FTH1 in spermato-genic cells,and that yeast β-glucan had an attenuating effect on ZEA-induced testicular cell ferrop-tosis in mice.关键词
玉米赤霉烯酮/铁死亡/β-葡聚糖/睾丸细胞/小鼠Key words
ZEA/ferroptosis/beta-glucan/testicular cells/mice分类
农业科技引用本文复制引用
赵子惠,李佳容,常倩雯,朱琪,张亚宁,胡婉星,贺俊平..酵母β-葡聚糖对玉米赤霉烯酮诱导小鼠睾丸细胞铁死亡的缓解作用[J].中国兽医科学,2024,54(10):1408-1416,9.基金项目
山西省现代农业牛产业技术体系项目(2023CYJSTX13-03) （2023CYJSTX13-03）
山西农业大学农业发展科技引领项目(CYYL-08) （CYYL-08）
