中国造纸学报2024，Vol.39Issue(3)：38-44,7.DOI:10.11981/j.issn.1000-6842.2024.03.38
可提高漆酶酶活的载锰活性炭-纤维素复合吸附剂的制备及应用
Preparation and Application of Manganese-loaded Activated Carbon-Cellulose Composite Adsorbent for Enhancing Laccase Enzyme Activity
摘要
Abstract
A novel manganese-loaded activated carbon-cellulose composite adsorbent that could immobilize laccase and increase the enzyme activity was prepared by solvent regeneration method,using waste manganese-loaded activated carbon and cellulose,and further prepared the laccase-carrier material after immobilizing laccase.The results showed that the content of Mn2+had a certain effect on the enzyme activity of the laccase-carrier material,and the enzyme activity of the laccase-carrier material was the highest when the content of Mn2+in the composite adsorbent was 1.5 mg/g.The optimum process conditions for the immobilization of laccase were the immobilization temperature of 45℃,the pH value of 4,and the initial enzyme concentration of 6.5 g/L.The immobilization ratio of the laccase-carrier material was the highest to 48.24%under the optimum conditions of immobilization;the thermal and pH stabilities of the laccase-carrier material were significantly en-hanced;the degradation rate of bisphenol A using the laccase-carrier material reached 68%,and the relative degradation rate of bisphenol A was still up to 51%after 5 times of reuse.关键词
废弃载锰活性炭/纤维素基吸附剂/固定化漆酶/双酚A降解Key words
waste manganese-loaded activated carbon/cellulose-based adsorbent/immobilized laccase/bisphenol A degradation分类
轻工纺织引用本文复制引用
李娜,孟祥锐,彭传博,于方睿,张健,盛雪茹,平清伟..可提高漆酶酶活的载锰活性炭-纤维素复合吸附剂的制备及应用[J].中国造纸学报,2024,39(3):38-44,7.基金项目
辽宁省教育厅面上项目(LJKMZ20220885) （LJKMZ20220885）
生物基材料与绿色造纸国家重点实验室开放基金项目(GZKF202225) （GZKF202225）
天津市制浆造纸重点实验室开放基金项目(202106). （202106）
