中国造纸学报2024，Vol.39Issue(3)：87-95,9.DOI:10.11981/j.issn.1000-6842.2024.03.87
α-淀粉酶及其复合酶体系对淀粉酶解性能研究
Study on the Starch Enzymolysis Performance of α-Amylase and Its Complex Enzyme System
摘要
Abstract
This study investigated the enzymatic performance of single α-amylase and binary,ternary,and quaternary complex enzyme systems consisting of α-amylase with β-amylase,pullulanase and glucoamylase for three commonly used modified starches(oxidized starch,enzymati-cally hydrolyzed starch,and cationic starch).The effect of different α-amylase dosages on starch enzymatic rate and DE value was explored.The results indicated that when the starch enzymatic rate reached 90%in single α-amylase system,the required amount of α-amylase for cat-ionic starch enzymolysis was about 4 times than that of the other two kinds of starches.The enzymatic rate of enzymatically hydrolyzed starch could be increased by up to 37.4%when citric acid was added to inhibit zinc ions.In the binary complex enzyme system,the enzymatic rate of cationic starch by α-amylase/β-amylase was 5.3%and 8.7%higher than that by α-amylase/glucoamylase and α-amylase/pullulanase,re-spectively.In the ternary complex enzyme system,the enzymatic rate of cationic starch by α-amylase/glucoamylase/β-amylase was 1.2%higher than that by α-amylase/glucoamylase/pullulanase.In the application of α-amylase/glucoamylase/β-amylase/pullulanase tetrameric com-plex enzyme system to the enzymatic reactions of cationic starch,enzymatically hydrolyzed starch and oxidized starch,when the enzymatic rate of starch reached 80%,the required amount of α-amylase was 56%,38%and 17%of that by single α-amylase system,respectively.关键词
变性淀粉/α-淀粉酶/复合酶体系/酶解效率Key words
modified starch/α-amylase/complex enzymes/enzymatic hydrolysis efficiency分类
轻工纺织引用本文复制引用
朱鹤馨,汪思维,胡慧捷,赵铭,刘念,胡静,蒋云,邓海波..α-淀粉酶及其复合酶体系对淀粉酶解性能研究[J].中国造纸学报,2024,39(3):87-95,9.基金项目
国家十四五重点研发计划(2022YFC2105504). （2022YFC2105504）
